Characterization of cDNA and the genomic sequence encoding canine neural-cell adhesion molecule, CD56/N-CAM

The neural-cell adhesion molecule, CD56/N-CAM is a member of the immunoglobulin superfamily expressed by various tissues and cells, including natural killer (NK) cells. Despite the importance of CD56 as a marker for identifying NK cells in circulating blood, canine CD56 has not been identified. In t...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2005-08, Vol.107 (1), p.171-176
Main Authors: Bonkobara, M., Sato, T., Takahashi, N., Kasahara, Y., Yagihara, H., Tamura, K., Isotani, M., Azakami, D., Ono, K., Washizu, T.
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Animals
Base Sequence
CD56 Antigen - chemistry
CD56 Antigen - genetics
CD56/N-CAM
cDNA cloning
DNA, Complementary - genetics
Dog
Dogs - genetics
Dogs - immunology
Genome
Humans
Molecular Sequence Data
Molecular Weight
Phylogeny
Protein Isoforms - chemistry
Protein Isoforms - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Species Specificity
Tissue Distribution
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Summary:The neural-cell adhesion molecule, CD56/N-CAM is a member of the immunoglobulin superfamily expressed by various tissues and cells, including natural killer (NK) cells. Despite the importance of CD56 as a marker for identifying NK cells in circulating blood, canine CD56 has not been identified. In the present study, we identified the canine counterparts of the 140-kDa (CD56-140) and 120-kDa (CD56-120) isoforms of human DC56. Both of amino acid sequences encoded by the canine CD56-140 and -120 cDNA showed high homology with those of human (both 96% homology), having well-conserved domains (five immunoglobulin, two fibronectin type III, and transmembrane and intracelluer or glycosylphosphatidylinositol-linked domain) among various species (human, mouse, and feline). We revealed that the transcripts of canine CD56-140 and -120 arise from alternative mRNA splicing from a single gene located on canine chromosome 5. Moreover, the mRNA encoding canine CD56-140 was expressed at high levels constitutively by nervous system and endocrine tissues as has shown in other animals.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2005.04.008