Insights into cyclodextrin interactions during sample stacking using capillary isotachophoresis with on-line microcoil NMR detection

On‐line capillary isotachophoresis (cITP)–NMR experiments were used to probe the interactions of the pharmaceutical compounds S‐alprenolol, S‐atenolol, R‐propranolol, R‐salbutamol and S‐terbutaline with β‐cyclodextrin (β‐CD) during cITP concentration. In cITP, ionic analytes are concentrated and sep...

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Bibliographic Details
Published in:Magnetic resonance in chemistry 2005-09, Vol.43 (9), p.755-761
Main Authors: Almeida, Valentino K., Larive, Cynthia K.
Format: Article
Language:English
Subjects:
Albuterol - analysis
Albuterol - chemistry
Alprenolol - analysis
Alprenolol - chemistry
Atenolol - analysis
Atenolol - chemistry
capillary isotachophoresis
Cyclodextrins - analysis
Cyclodextrins - chemistry
Diffusion
Electrolytes - chemistry
Electrophoresis - methods
inclusion complex
Magnetic Resonance Spectroscopy - methods
microcoil NMR
Propranolol - analysis
Propranolol - chemistry
Protons
Sensitivity and Specificity
Terbutaline - analysis
Terbutaline - chemistry
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Summary:On‐line capillary isotachophoresis (cITP)–NMR experiments were used to probe the interactions of the pharmaceutical compounds S‐alprenolol, S‐atenolol, R‐propranolol, R‐salbutamol and S‐terbutaline with β‐cyclodextrin (β‐CD) during cITP concentration. In cITP, ionic analytes are concentrated and separated on the basis of their electrophoretic mobility. Because neutral molecules have an electrophoretic mobility of zero, they are normally not concentrated or separated in electrophoretic experiments like cITP. Most of the analytes studied were concentrated by cITP sample stacking by a factor of around 300. For analytes that formed a strong inclusion complex, β‐CD co‐concentrated during cITP sample stacking. However, once the focusing process was complete, a discrete diffusional boundary formed between the cITP‐focused analyte band and the leading and trailing electrolyte, which restricted diffusion into and out of the analyte band. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-1581
1097-458X
DOI:10.1002/mrc.1626