Isolation of CD24(high) and CD24(low/-) cells from MCF-7: CD24 expression is positively related with proliferation, adhesion and invasion in MCF-7

CD24 has been reported to have a role in metastasis of several malignancies including breast cancer. We investigated the effect of CD24 expression on biologic features in human breast cancer cells, and found that CD24 expression is associated with more rapid growth and enhanced ability of adhesion a...

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Bibliographic Details
Published in:Cancer letters 2007-12, Vol.258 (1), p.98-108
Main Authors: Kim, Hee Joung, Kim, Jong Bin, Lee, Kyung-Min, Shin, Incheol, Han, Wonshik, Ko, Eunyoung, Bae, Ji-Yeon, Noh, Dong-Young
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - pharmacology
Antineoplastic Agents, Hormonal - pharmacology
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
CD24 Antigen - genetics
CD24 Antigen - metabolism
Cell Adhesion
Cell Cycle - drug effects
Cell Line, Tumor
Cell Proliferation
Cyclin D
Cyclin-Dependent Kinase Inhibitor p27 - metabolism
Cyclins - metabolism
Doxorubicin - pharmacology
Estrogen Receptor alpha - metabolism
Female
Flow Cytometry
Humans
Mice
Neoplasm Invasiveness - pathology
Tamoxifen - pharmacology
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - metabolism
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Summary:CD24 has been reported to have a role in metastasis of several malignancies including breast cancer. We investigated the effect of CD24 expression on biologic features in human breast cancer cells, and found that CD24 expression is associated with more rapid growth and enhanced ability of adhesion and invasion in MCF-7. The proportion of cells was higher in synthetic phase and lower in arrestic phase for CD24(high) than for CD24(low/-) cells. Cyclin D1 and p27 had stronger expressions in CD24(low/-) cells than CD24(high) cells. These suggested one possible pathway for CD24 effect on proliferation.
ISSN:0304-3835
DOI:10.1016/j.canlet.2007.08.025