Towards tissue specific transcriptomics and expression pattern analysis in schistosomes using laser microdissection microscopy

One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is...

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Bibliographic Details
Published in:Experimental parasitology 2007-11, Vol.117 (3), p.259-266
Main Authors: Jones, Malcolm K., Higgins, Tess, Stenzel, Deborah J., Gobert, Geoffrey N.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling - methods
Invertebrates
laser microdissection microscopy
Life cycle. Host-agent relationship. Pathogenesis
LMM
Microdissection - instrumentation
Microscopy, Confocal - methods
Nemathelminthia. Plathelmintha
Proteomics
Proteomics - methods
Protozoa
Schistosoma
Schistosoma - genetics
Schistosoma - physiology
Schistosoma - ultrastructure
Transcriptomics
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Summary:One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This technique allows tissues to be dissected for subsequent molecular and protein analysis. The method is reviewed in the light of the acoelomate triploblastic nature of tissue organisation in the parasite.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2007.06.004