Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z [almost equal to] 29,000 peak, which was confirmed as β-lactamase...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2007-11, Vol.389 (5), p.1633-1638
Main Authors: Camara, Johanna E, Hays, Faith A
Format: Article
Language:English
Subjects:
Ampicillin
antibiotic resistance
beta-Lactamases - analysis
Drug Resistance
Escherichia coli
Escherichia coli - isolation & purification
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry - methods
β-Lactamase
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Summary:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z [almost equal to] 29,000 peak, which was confirmed as β-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-007-1558-7