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Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus

The white-rot basidiomycete Physisporinus rivulosus strain T241i is highly selective for degradation of softwood lignin, which makes this fungus suitable for biopulping. In order to promote laccase production, P. rivulosus was cultivated in nutrient-nitrogen sufficient liquid media containing either...

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Published in:Applied microbiology and biotechnology 2007-11, Vol.77 (2), p.301-309
Main Authors: Hildén, Kristiina, Hakala, Terhi K, Maijala, Pekka, Lundell, Taina K, Hatakka, Annele
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description The white-rot basidiomycete Physisporinus rivulosus strain T241i is highly selective for degradation of softwood lignin, which makes this fungus suitable for biopulping. In order to promote laccase production, P. rivulosus was cultivated in nutrient-nitrogen sufficient liquid media containing either charcoal or spruce sawdust as supplements. Two laccases with distinct pI values, Lac-3.5 and Lac-4.8, were purified from peptone-spruce sawdust-charcoal cultures of P. rivulosus. Both laccases showed thermal stability at up to 60°C. Lac-4.8 was thermally activated at 50°C. Surprisingly, both laccases displayed atypically low pH optima (pH 3.0-3.5) in oxidation of the commonly used laccase substrates syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine), 2,6-dimethoxyphenol and guaiacol (2-methoxyphenol). Steady-state kinetic measurements pointed to unusually low affinity to guaiacol at low pH, whereas the kinetic constants for the methoxyphenols and ABTS were within the ranges reported for other fungal laccases. The combination of thermotolerance with low pH optima for methoxylated phenol substrates suggests that the two P. rivulosus T241i laccases possess potential for use in biotechnological applications.
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source ABI/INFORM Global; Springer Nature
subjects Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Carbon
Charcoal
Culture Media
Energy consumption
Enzyme kinetics
Enzyme Stability
Fundamental and applied biological sciences. Psychology
Fungi
Glucose
Hot Temperature
Industrial Microbiology - methods
Isoenzymes - biosynthesis
Kinetics
laccase
Laccase - biosynthesis
Lignin
Lignin - metabolism
Lignin degradation
Miscellaneous
Mission oriented research
Nitrogen
Oxidation
Peptones
Phenols
Physisporinus rivulosus
Picea - metabolism
Polyporaceae - enzymology
Polyporaceae - genetics
Polyporaceae - growth & development
Proteins
Sawdust
Sodium
Softwoods
Studies
Thermal activation
thermal stability
Trees
white-rot fungi
Wood - metabolism
Yeast
title Novel thermotolerant laccases produced by the white-rot fungus Physisporinus rivulosus
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