Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores

A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthraci...

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Bibliographic Details
Published in:Biotechnology letters 2007-12, Vol.29 (12), p.1939-1946
Main Authors: Qiao, Yan-Mei, Guo, Yong-Chao, Zhang, Xian-En, Zhou, Ya-Feng, Zhang, Zhi-Ping, Wei, Hong-Ping, Yang, Rui-Fu, Wang, Dian-Bing
Format: Article
Language:English
Subjects:
Bacillus anthracis
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacteria
Biological and medical sciences
Biotechnology
detection
Detection limits
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Isothermal amplification
LAMP
Microbiology
Nucleic Acid Amplification Techniques - methods
Powders
Restriction Mapping
Spores, Bacterial - genetics
Spores, Bacterial - isolation & purification
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Summary:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-007-9472-9