Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores

A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthraci...

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Published in:Biotechnology letters 2007-12, Vol.29 (12), p.1939-1946
Main Authors: Qiao, Yan-Mei, Guo, Yong-Chao, Zhang, Xian-En, Zhou, Ya-Feng, Zhang, Zhi-Ping, Wei, Hong-Ping, Yang, Rui-Fu, Wang, Dian-Bing
Format: Article
Language:English
Subjects:
Bacillus anthracis
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacteria
Biological and medical sciences
Biotechnology
detection
Detection limits
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Isothermal amplification
LAMP
Microbiology
Nucleic Acid Amplification Techniques - methods
Powders
Restriction Mapping
Spores, Bacterial - genetics
Spores, Bacterial - isolation & purification
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cited_by cdi_FETCH-LOGICAL-c446t-b13308dce563927b76b64592c7efa821e08047169eeb313d2e8206fe0ffa5e6b3
cites cdi_FETCH-LOGICAL-c446t-b13308dce563927b76b64592c7efa821e08047169eeb313d2e8206fe0ffa5e6b3
container_end_page 1946
container_issue 12
container_start_page 1939
container_title Biotechnology letters
container_volume 29
creator Qiao, Yan-Mei
Guo, Yong-Chao
Zhang, Xian-En
Zhou, Ya-Feng
Zhang, Zhi-Ping
Wei, Hong-Ping
Yang, Rui-Fu
Wang, Dian-Bing
description A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.
doi_str_mv 10.1007/s10529-007-9472-9
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ispartof Biotechnology letters, 2007-12, Vol.29 (12), p.1939-1946
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source Springer Nature
subjects Bacillus anthracis
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacteria
Biological and medical sciences
Biotechnology
detection
Detection limits
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electrophoresis, Agar Gel
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Isothermal amplification
LAMP
Microbiology
Nucleic Acid Amplification Techniques - methods
Powders
Restriction Mapping
Spores, Bacterial - genetics
Spores, Bacterial - isolation & purification
title Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores
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