Characterization of Phenolic Profiles of Northern European Berries by Capillary Electrophoresis and Determination of their Antioxidant Activity

• Published in: Journal of agricultural and food chemistry 2005-08, Vol.53 (16), p.6484-6490
• Main Authors: Ehala, Sille, Vaher, Merike, Kaljurand, Mihkel
• Format: Article
• Language: English
• Subjects: Antioxidants - analysis; Electrophoresis, Capillary; Europe; Fragaria - chemistry; Fruit - chemistry; Phenols - analysis; Ribes - chemistry; Vaccinium - chemistry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf050397w

Berries are known to contain phenolic substances (i.e., flavonoids and phenolic acids), which comprise two large and heterogeneous groups of biologically active nonnutrients. This investigation evaluated the content and profile of the phenolic compounds present in six different berries found in Northern Europe. The latter included bilberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), strawberry (Fragaria ananassa), black currant (Ribes nigrum), and red currant (Ribes rubrum). The study was focused on two areas. The first involved the extraction and analysis of berries for total phenolic content and determination of their antioxidant activity. The total phenolic level of berries was correlated with their antioxidant activity. Second, the berry extracts were analyzed by capillary electrophoresis to determine the content and profile of selected bioactive compounds. The analytes of interest included trans-resveratrol, cinnamic acid, ferulic acid, p-coumaric acid, quercetin, and morin. Keywords: Berries; polyphenols; capillary electrophoresis; antioxidant activity