Differential expression of erythroid genes in prion disease

• Published in: Biochemical and biophysical research communications 2007-12, Vol.364 (2), p.366-371
• Main Authors: Brown, A.R., Blanco, A.R. Alejo, Miele, G., Hawkins, S.A., Hopkins, J., Fazakerley, J.K., Manson, J., Clinton, M.
• Format: Article
• Language: English
• Subjects: Alphavirus Infections - metabolism; Animals; Ankyrin; Band 3; Biomarkers - metabolism; Bovine spongiform encephalopathy; Cardiovirus Infections - metabolism; Cattle; Disease Models, Animal; Encephalopathy, Bovine Spongiform - metabolism; ERAF; Erythroid; Erythroid Cells - metabolism; Female; Gammaherpesvirinae; Gene Expression Profiling; Glycophorin A; Herpesviridae Infections - metabolism; Kell; Male; Mice; Mice, Inbred Strains; Prion; Prion Diseases - metabolism; Scrapie; Scrapie - metabolism; Semliki forest virus; Sheep; Theilovirus
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.10.015

We previously reported reduced expression of erythroid-associated factor (ERAF) within haematopoietic tissues of rodent scrapie models, suggesting an unrecognized role for the erythroid lineage in prion disease. In the present study, we compared the expression of a panel of erythroid genes within four murine scrapie models and five virus infection models with parallels to prion disease pathogenesis. We report that differential expression of erythroid genes is not limited to ERAF, and is a common feature of murine scrapie, dependent on host expression of cellular prion protein. In contrast, erythroid gene expression was not altered following virus infection. Whilst these results further implicate cells of the erythroid lineage in the peripheral pathogenesis of prion disease, analysis of blood from BSE-infected cattle and scrapie-infected sheep reveals that the extent of differential expression of erythroid genes within peripheral blood is not sufficient to provide a discriminatory diagnostic test.