Ionic liquids as a reaction medium for lipase-catalyzed methanolysis of sunflower oil

Ionic liquids, 1-butyl-3-methyl imidazolium hexafluorophosphate ([BMIm][PF₆]) and 1-ethyl-3-methyl imidazolium hexafluorophosphate ([EMIm][PF₆]), were used for the methanolysis of sunflower oil using Candida antarctica lipase (Novozyme 435) and gave yields of fatty acid methyl esters at 98-99% withi...

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Bibliographic Details
Published in:Biotechnology letters 2007-12, Vol.29 (12), p.1881-1885
Main Authors: Sunitha, S, Kanjilal, S, Reddy, P. S, Prasad, R. B. N
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Candida - enzymology
Catalysis
Esters
Fatty acid methyl ester
Fundamental and applied biological sciences. Psychology
Hydrophobic and Hydrophilic Interactions
Ionic liquid
Ionic Liquids - metabolism
Ions
Lipase - metabolism
Methanol
Methanol - metabolism
Methanolysis
Plant Oils - metabolism
Solvents
Sunflower Oil
Time Factors
triacylglycerol lipase
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Summary:Ionic liquids, 1-butyl-3-methyl imidazolium hexafluorophosphate ([BMIm][PF₆]) and 1-ethyl-3-methyl imidazolium hexafluorophosphate ([EMIm][PF₆]), were used for the methanolysis of sunflower oil using Candida antarctica lipase (Novozyme 435) and gave yields of fatty acid methyl esters at 98-99% within 10 h. The optimum conditions of methanolysis in hydrophobic ionic liquids are 2% (w/w) lipase, 1:1 (w/w) oil/ionic liquid and 1:8 (mol/mol) oil/methanol at 58-60°C. Methanolysis using hydrophilic ionic liquids, 3-methyl imidazolium tetrafluoroborate ([HMIm][BF₄]) and 1-butyl-3-methyl imidazolium tetrafluoroborate ([BMIm][BF₄]), gave very poor yields. A hydrophobic ionic liquid thus protects the lipase from methanol. Recovered ionic liquids and lipase were used for four successive reaction cycles without any significant loss of activity.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-007-9471-x