Interest of new molecular typing method in the study of hospital transmitted Staphylococcus aureus population

• Published in: Pathologie biologie (Paris) 2007-11, Vol.55 (8-9), p.382-389
• Main Authors: Védy, S, Garnotel, E, Koeck, J-L, Simon, F, Molinier, S, Puidupin, A
• Format: Article
• Language: fre
• Subjects: Cross Infection - classification; France; Genotype; Hospitalization - statistics & numerical data; Humans; Models, Biological; Risk Factors; Staphylococcal Infections - epidemiology; Staphylococcal Infections - transmission; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - pathogenicity; Urban Population
• ISSN: 0369-8114
• DOI: 10.1016/j.patbio.2007.06.005

To determinate the origin of acquired S. aureus among hospitalised patients and to evaluate the transmission of strains between health care workers and hopistalised patients. The method chosen is a prospective study in risky clinical yards. Nasal swabing of patients and health care workers has been done to isolate bacterial samples. Caracterisation and comparaison of bacterial strains have been made using their antibiotic resistance profil and a recent molecular genotyping technic named MLVA (Multi Locus Variable Number of Tandem Repeat). It has never been used in such context. One hundred and fifty-seven strains have been isolated. They have been compared while realizing 1900 PCR and agar gel electrophoresis in 10 days. 15 clones were identified. One of them is mainly represented among patient's nasal carriage and acquired strains. As far as antibiotype and agr type are concerned, it is similar to hospital-acquired clone described in Europe with other technics (MRSA, Gentamicine-S agr 1). This clone appears to be also transmitted between health care workers and patients. Although it exists, we can't appreciate the intensity of this transmission. These results don't allow us to proceed to a systematic screening for nasal carriage among our health care workers. This study shows that MLVA could be a reliable molecular typing method, which could be used in every day practice. In our experience, it is as performing as PFGE, more didactic, faster and easier.