The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium

To investigate whether bone marrow cells migrate and are engrafted into the olfactory epithelium and differentiate into olfactory neurons, bone marrow cells of green fluorescence protein (GFP) mice were transplanted into lethally irradiated recipient mice. Immunohistochemical staining was performed...

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Bibliographic Details
Published in:Brain research 2005-08, Vol.1052 (1), p.10-15
Main Authors: Tsujigiwa, Hidetsugu, Nishizaki, Kazunori, Teshima, Takanori, Takeda, Yasushi, Yoshinobu, Junko, Takeuchi, Ayako, Orita, Yorihisa, Sugata, Yuji, Nagatsuka, Hitoshi, Nagai, Noriyuki
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biological and medical sciences
Bone marrow
Bone Marrow Transplantation
Cell Count - methods
Cell Differentiation - physiology
Cell Movement - physiology
Engraftment
Female
Fundamental and applied biological sciences. Psychology
GFP mice
Green Fluorescent Proteins - biosynthesis
Immunohistochemistry - methods
Indoles
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nerve Tissue Proteins - metabolism
Neurons - physiology
Olfactory epithelium
Olfactory Marker Protein
Olfactory Mucosa - cytology
Olfactory Mucosa - physiology
Olfactory system and olfaction. Gustatory system and gustation
Transplantation
Vertebrates: nervous system and sense organs
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Summary:To investigate whether bone marrow cells migrate and are engrafted into the olfactory epithelium and differentiate into olfactory neurons, bone marrow cells of green fluorescence protein (GFP) mice were transplanted into lethally irradiated recipient mice. Immunohistochemical staining was performed to evaluate the engraftment of donor bone marrow cells into the olfactory epithelium. Immunostaining for GFP was found initially in the olfactory epithelium 2 weeks after bone marrow reconstruction. The percentage of GFP positive cells increased up to 12 months after bone marrow reconstruction. Double staining for GFP and olfactory marker protein showed that a population of the GFP-positive cells had characteristics of olfactory neurons. These results demonstrate that bone marrow cells can be engrafted in the olfactory epithelium and then differentiate into olfactory neuron cells.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2005.05.061