RNA interference-mediated silencing of the syntaxin 5 gene induces Golgi fragmentation but capable of transporting vesicles

• Published in: FEBS letters 2005-08, Vol.579 (20), p.4226-4234
• Main Authors: Suga, Kei, Hattori, Hiroshi, Saito, Ayako, Akagawa, Kimio
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; ELISA; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; enzyme-linked immunosorbent assay; ERGIC/VTC; ER–Golgi-intermediate compartment/vesicular tubular carriers; GFP; glutathione-S-transferase; Golgi; Golgi Apparatus - metabolism; Golgi Apparatus - ultrastructure; green fluorescent protein; GST; hemagglutinin; Humans; mAb; Membrane Proteins - antagonists & inhibitors; Membrane Proteins - genetics; Membrane Proteins - physiology; Molecular Sequence Data; Monoclonal antibody; Protein Transport; Qa-SNARE Proteins; RNA Interference; RNAi; siRNA; small interfering RNA; SNARE; soluble N-ethylmaleimide-sensitive factor-attachment protein receptor; Syntaxin; Syx; Transport Vesicles - metabolism; Vesicular stomatitis Indiana virus - metabolism; Vesicular stomatitis virus; vesicular stomatitis virus ts045 G; Vesicular transport; Viral Proteins - metabolism; VSVG
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2005.06.053

It has been suggested that syntaxin 5 (Syx5) participates in vesicular transport. We examined the effects of Syx5 down-regulation on the morphology of the Golgi apparatus and the transport of vesicles in mammalian cells. Knockdown of the Syx5 gene resulted in Golgi fragmentation without changing the level of endoplasmic reticulum (ER)-resident proteins, other Golgi-SNAREs (soluble N-ethylmaleimide-sensitive factor-attachment protein receptors), and coatmer proteins. Strikingly, a major decrease in Syx5 expression barely affected the anterograde transport of vesicular stomatitis virus G (VSVG) protein to the plasma membrane. These results suggest that Syx5 is required for the maintenance of the Golgi structures but may not play a major role in the transport of vesicles carrying VSVG between the ER and the Golgi compartment.