Tobamovirus-resistant tobacco generated by RNA interference directed against host genes

Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and...

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Bibliographic Details
Published in:FEBS letters 2005-08, Vol.579 (20), p.4479-4484
Main Authors: Asano, Momoko, Satoh, Rena, Mochizuki, Atsuko, Tsuda, Shinya, Yamanaka, Takuya, Nishiguchi, Masamichi, Hirai, Katsuyuki, Meshi, Tetsuo, Naito, Satoshi, Ishikawa, Masayuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis thaliana
CaMV
Cauliflower mosaic virus
CMV
coat protein
Cucumber mosaic virus
days post-inoculation
dpi
Genes, Plant - genetics
Molecular Sequence Data
Mutation
Nicotiana - genetics
Nicotiana - virology
Nicotiana tabacum
Pepper mottle mosaic virus
Plant Diseases - genetics
Plant Diseases - virology
PMMoV
Resistance
RNA Interference
RNAi
TMGMV
TMV
Tobacco mild green mosaic virus
Tobacco mosaic virus
Tobamovirus
Tobamovirus - genetics
Tobamovirus - physiology
TOM1
Tomato mosaic virus
ToMV
Virus Replication - genetics
Virus Replication - physiology
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Summary:Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus-resistant plants.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.07.021