Serological identification of breast cancer‐related antigens from a Saccharomyces cerevisiae surface display library

• Published in: International journal of cancer 2005-10, Vol.117 (1), p.104-113
• Main Authors: Wadle, Andreas, Mischo, Axel, Imig, Jochen, Wüllner, Beate, Hensel, Dan, Wätzig, Kristin, Neumann, Frank, Kubuschok, Boris, Schmidt, Werner, Old, Lloyd J., Pfreundschuh, Michael, Renner, Christoph
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antigens, Neoplasm - blood; Antigens, Neoplasm - genetics; Antigens, Neoplasm - immunology; Antigens, Surface - immunology; Base Sequence; Biological and medical sciences; Breast Neoplasms - blood; Breast Neoplasms - genetics; Breast Neoplasms - immunology; cDNA expression library; Cloning, Molecular; DNA, Complementary - genetics; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression Regulation, Neoplastic; Gene Library; Gynecology. Andrology. Obstetrics; Humans; Mammary gland diseases; Mammary Glands, Human - metabolism; Medical sciences; Membrane Proteins - genetics; Membrane Proteins - immunology; Membrane Proteins - metabolism; Molecular Sequence Data; Mucins - genetics; Mucins - immunology; Mucins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - immunology; Saccharomyces cerevisiae - metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Serologic Tests; serological screening; tumor antigens; Tumors; yeast surface display
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/ijc.21147

A yeast cell surface display technology was used for the isolation and characterization of tumor antigens recognised by autologous or allogeneic breast cancer serum. More than 100 clones recognized by patient serum were isolated using high‐through‐put fluorescence activated cell sorting. Combined serological and sequence analysis confirmed that a number of proteins known to be overexpressed in breast cancer tissue could be detected. A recently identified small breast epithelial mucin almost exclusively expressed in mammary gland tissue was isolated as a mutated protein variant. Subsequent serological analysis using the yeast expression system for the wild‐type and mutant form showed a strong recognition by patient sera, whereas no significant recognition was observed for the respective prokaryotically expressed proteins. The small breast epithelial mucin is present to a large extent in a membrane bound format and might be used for tumor targeting strategies. © 2005 Wiley‐Liss, Inc.