Thermodynamic Stability of RNA Structures Formed by CNG Trinucleotide Repeats. Implication for Prediction of RNA Structure

Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligor...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 2005-08, Vol.44 (32), p.10873-10882
Main Authors: Broda, Magdalena, Kierzek, Elżbieta, Gdaniec, Zofia, Kulinski, Tadeusz, Kierzek, Ryszard
Format: Article
Language:English
Subjects:
Models, Molecular
Nucleic Acid Conformation
RNA - chemistry
RNA Stability
Thermodynamics
Trinucleotide Repeats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides composed of two to seven of CAG, CCG, CGG, and CUG repeats. The thermodynamic parameters of helix propagation correlated with the presence of multiple N-N mismatches within CNG RNA duplexes were also determined. Moreover, the total stability of CNG RNA hairpins, as well as the contribution of trinucleotide repeats placed only in the stem or loop regions, was evaluated. The improved thermodynamic parameters allow to predict much more accurately the thermodynamic stabilities and structures of CNG RNAs.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi0502339