Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model

Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combi...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-08, Vol.5 (12), p.3173-3182
Main Authors: Wu, Chih-Ching, Chien, Kun-Yi, Tsang, Ngan-Ming, Chang, Kai-Ping, Hao, Sheng-Po, Tsao, Chuang-Hui, Chang, Yu-Sun, Yu, Jau-Song
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Biomarkers, Tumor - chemistry
Blotting, Western
Carcinoma - metabolism
Cell Line, Tumor
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fibronectins - metabolism
Fundamental and applied biological sciences. Psychology
Galectin 3 - metabolism
Humans
Immunohistochemistry
Mass Spectrometry
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Nude
Miscellaneous
Nasopharyngeal carcinoma
Nasopharyngeal Neoplasms - metabolism
Neoplasm Transplantation
Otorhinolaryngology. Stomatology
Plasminogen Activator Inhibitor 1 - metabolism
Proteins
Proteomics - methods
Secreted proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tumor markers
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combined with MALDI‐TOF MS. Twenty‐three proteins were found in cultured media from both NPC cell lines. Among them, fibronectin, Mac‐2 binding protein (Mac‐2 BP), and plasminogen activator inhibitor 1 (PAI‐1) were further confirmed by Western blot analysis. These three proteins were highly expressed in NPC biopsies, but weakly or not expressed in normal nasopharyngeal tissues. The serum levels of the three proteins were significantly higher in NPC patients (n = 46) than in normal controls (n = 47) (p 
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200401133