Loading…
Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model
Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combi...
Saved in:
| Published in: | Proteomics (Weinheim) 2005-08, Vol.5 (12), p.3173-3182 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93 |
| container_end_page | 3182 |
| container_issue | 12 |
| container_start_page | 3173 |
| container_title | Proteomics (Weinheim) |
| container_volume | 5 |
| creator | Wu, Chih-Ching Chien, Kun-Yi Tsang, Ngan-Ming Chang, Kai-Ping Hao, Sheng-Po Tsao, Chuang-Hui Chang, Yu-Sun Yu, Jau-Song |
| description | Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combined with MALDI‐TOF MS. Twenty‐three proteins were found in cultured media from both NPC cell lines. Among them, fibronectin, Mac‐2 binding protein (Mac‐2 BP), and plasminogen activator inhibitor 1 (PAI‐1) were further confirmed by Western blot analysis. These three proteins were highly expressed in NPC biopsies, but weakly or not expressed in normal nasopharyngeal tissues. The serum levels of the three proteins were significantly higher in NPC patients (n = 46) than in normal controls (n = 47) (p |
| doi_str_mv | 10.1002/pmic.200401133 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68464986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68464986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EoqVw5Yh8gVuWcWzHCTdYoBSVAgIE4mI5zqQ1zReerGD_e7zKasutkiVbmt-bmefH2GMBKwGQP5_64Fc5gAIhpLzDjkUhdFaVhbh7eGt5xB4Q_QIQpqzMfXYkCpBaCHHM5rUbPEbusesyQh9xxoZPcZxx7JG4S4fXjgLxdoyc0EV_FYZLPiVimIPr-LzpU6V38RojveAXjsbpysXtcImp6pMgDGPvllb92GD3kN1rXUf4aH-fsG9v33xdv8vOP56erV-eZ16pXGZlVWJZo8t9jlIBOgCE0ldQy8Jj7dEpgEJr47H10CotUSGaJsdGa-UrecKeLX2Tn98bpNn2gXZO3YDjhmxRqkKlv7oVFEZXUpUqgasF9HEkitjaKYZkfWsF2F0gdheIPQSSBE_2nTd1j80Nvk8gAU_3gCPvujamPALdcAbA5GY3uVq4P6HD7S1j7acPZ-v_l8gWbaAZ_x60KTFbGGm0_X5xar-8Uq8__yze2x_yHxNstZ8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17593484</pqid></control><display><type>article</type><title>Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model</title><source>Wiley</source><creator>Wu, Chih-Ching ; Chien, Kun-Yi ; Tsang, Ngan-Ming ; Chang, Kai-Ping ; Hao, Sheng-Po ; Tsao, Chuang-Hui ; Chang, Yu-Sun ; Yu, Jau-Song</creator><creatorcontrib>Wu, Chih-Ching ; Chien, Kun-Yi ; Tsang, Ngan-Ming ; Chang, Kai-Ping ; Hao, Sheng-Po ; Tsao, Chuang-Hui ; Chang, Yu-Sun ; Yu, Jau-Song</creatorcontrib><description>Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combined with MALDI‐TOF MS. Twenty‐three proteins were found in cultured media from both NPC cell lines. Among them, fibronectin, Mac‐2 binding protein (Mac‐2 BP), and plasminogen activator inhibitor 1 (PAI‐1) were further confirmed by Western blot analysis. These three proteins were highly expressed in NPC biopsies, but weakly or not expressed in normal nasopharyngeal tissues. The serum levels of the three proteins were significantly higher in NPC patients (n = 46) than in normal controls (n = 47) (p < 0.01). NPC nude mice model (n = 9) also showed elevated levels of serum Mac‐2 BP and PAI‐1 compared with tumor‐free mice (n = 9) (p < 0.01). Systematic analysis of cancer cell‐secreted proteomes combined with animal tumor models can be a feasible, convenient strategy for searching multiple potential tumor markers. Furthermore, our work shows that fibronectin, Mac‐2 BP, and PAI‐1 may be potential markers for diagnosis of NPC.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.200401133</identifier><identifier>PMID: 16035111</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Biomarkers, Tumor - chemistry ; Blotting, Western ; Carcinoma - metabolism ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Galectin 3 - metabolism ; Humans ; Immunohistochemistry ; Mass Spectrometry ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Miscellaneous ; Nasopharyngeal carcinoma ; Nasopharyngeal Neoplasms - metabolism ; Neoplasm Transplantation ; Otorhinolaryngology. Stomatology ; Plasminogen Activator Inhibitor 1 - metabolism ; Proteins ; Proteomics - methods ; Secreted proteins ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tumor markers ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Proteomics (Weinheim), 2005-08, Vol.5 (12), p.3173-3182</ispartof><rights>Copyright © 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93</citedby><cites>FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17007274$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16035111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Chih-Ching</creatorcontrib><creatorcontrib>Chien, Kun-Yi</creatorcontrib><creatorcontrib>Tsang, Ngan-Ming</creatorcontrib><creatorcontrib>Chang, Kai-Ping</creatorcontrib><creatorcontrib>Hao, Sheng-Po</creatorcontrib><creatorcontrib>Tsao, Chuang-Hui</creatorcontrib><creatorcontrib>Chang, Yu-Sun</creatorcontrib><creatorcontrib>Yu, Jau-Song</creatorcontrib><title>Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combined with MALDI‐TOF MS. Twenty‐three proteins were found in cultured media from both NPC cell lines. Among them, fibronectin, Mac‐2 binding protein (Mac‐2 BP), and plasminogen activator inhibitor 1 (PAI‐1) were further confirmed by Western blot analysis. These three proteins were highly expressed in NPC biopsies, but weakly or not expressed in normal nasopharyngeal tissues. The serum levels of the three proteins were significantly higher in NPC patients (n = 46) than in normal controls (n = 47) (p < 0.01). NPC nude mice model (n = 9) also showed elevated levels of serum Mac‐2 BP and PAI‐1 compared with tumor‐free mice (n = 9) (p < 0.01). Systematic analysis of cancer cell‐secreted proteomes combined with animal tumor models can be a feasible, convenient strategy for searching multiple potential tumor markers. Furthermore, our work shows that fibronectin, Mac‐2 BP, and PAI‐1 may be potential markers for diagnosis of NPC.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - chemistry</subject><subject>Blotting, Western</subject><subject>Carcinoma - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galectin 3 - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Mass Spectrometry</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Miscellaneous</subject><subject>Nasopharyngeal carcinoma</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Neoplasm Transplantation</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Plasminogen Activator Inhibitor 1 - metabolism</subject><subject>Proteins</subject><subject>Proteomics - methods</subject><subject>Secreted proteins</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Tumor markers</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkc1v1DAQxS0EoqVw5Yh8gVuWcWzHCTdYoBSVAgIE4mI5zqQ1zReerGD_e7zKasutkiVbmt-bmefH2GMBKwGQP5_64Fc5gAIhpLzDjkUhdFaVhbh7eGt5xB4Q_QIQpqzMfXYkCpBaCHHM5rUbPEbusesyQh9xxoZPcZxx7JG4S4fXjgLxdoyc0EV_FYZLPiVimIPr-LzpU6V38RojveAXjsbpysXtcImp6pMgDGPvllb92GD3kN1rXUf4aH-fsG9v33xdv8vOP56erV-eZ16pXGZlVWJZo8t9jlIBOgCE0ldQy8Jj7dEpgEJr47H10CotUSGaJsdGa-UrecKeLX2Tn98bpNn2gXZO3YDjhmxRqkKlv7oVFEZXUpUqgasF9HEkitjaKYZkfWsF2F0gdheIPQSSBE_2nTd1j80Nvk8gAU_3gCPvujamPALdcAbA5GY3uVq4P6HD7S1j7acPZ-v_l8gWbaAZ_x60KTFbGGm0_X5xar-8Uq8__yze2x_yHxNstZ8</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Wu, Chih-Ching</creator><creator>Chien, Kun-Yi</creator><creator>Tsang, Ngan-Ming</creator><creator>Chang, Kai-Ping</creator><creator>Hao, Sheng-Po</creator><creator>Tsao, Chuang-Hui</creator><creator>Chang, Yu-Sun</creator><creator>Yu, Jau-Song</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model</title><author>Wu, Chih-Ching ; Chien, Kun-Yi ; Tsang, Ngan-Ming ; Chang, Kai-Ping ; Hao, Sheng-Po ; Tsao, Chuang-Hui ; Chang, Yu-Sun ; Yu, Jau-Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - chemistry</topic><topic>Blotting, Western</topic><topic>Carcinoma - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galectin 3 - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Mass Spectrometry</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Miscellaneous</topic><topic>Nasopharyngeal carcinoma</topic><topic>Nasopharyngeal Neoplasms - metabolism</topic><topic>Neoplasm Transplantation</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Plasminogen Activator Inhibitor 1 - metabolism</topic><topic>Proteins</topic><topic>Proteomics - methods</topic><topic>Secreted proteins</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Tumor markers</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chih-Ching</creatorcontrib><creatorcontrib>Chien, Kun-Yi</creatorcontrib><creatorcontrib>Tsang, Ngan-Ming</creatorcontrib><creatorcontrib>Chang, Kai-Ping</creatorcontrib><creatorcontrib>Hao, Sheng-Po</creatorcontrib><creatorcontrib>Tsao, Chuang-Hui</creatorcontrib><creatorcontrib>Chang, Yu-Sun</creatorcontrib><creatorcontrib>Yu, Jau-Song</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chih-Ching</au><au>Chien, Kun-Yi</au><au>Tsang, Ngan-Ming</au><au>Chang, Kai-Ping</au><au>Hao, Sheng-Po</au><au>Tsao, Chuang-Hui</au><au>Chang, Yu-Sun</au><au>Yu, Jau-Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2005-08-01</date><risdate>2005</risdate><volume>5</volume><issue>12</issue><spage>3173</spage><epage>3182</epage><pages>3173-3182</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>Nasopharyngeal carcinoma (NPC) is commonly diagnosed late due to its deep location and vague symptoms. To identify biomarkers for early NPC diagnosis, secreted proteomes of two NPC cell lines were analyzed. Proteins in the NPC cell‐line cultured media were systematically identified by SDS‐PAGE combined with MALDI‐TOF MS. Twenty‐three proteins were found in cultured media from both NPC cell lines. Among them, fibronectin, Mac‐2 binding protein (Mac‐2 BP), and plasminogen activator inhibitor 1 (PAI‐1) were further confirmed by Western blot analysis. These three proteins were highly expressed in NPC biopsies, but weakly or not expressed in normal nasopharyngeal tissues. The serum levels of the three proteins were significantly higher in NPC patients (n = 46) than in normal controls (n = 47) (p < 0.01). NPC nude mice model (n = 9) also showed elevated levels of serum Mac‐2 BP and PAI‐1 compared with tumor‐free mice (n = 9) (p < 0.01). Systematic analysis of cancer cell‐secreted proteomes combined with animal tumor models can be a feasible, convenient strategy for searching multiple potential tumor markers. Furthermore, our work shows that fibronectin, Mac‐2 BP, and PAI‐1 may be potential markers for diagnosis of NPC.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>16035111</pmid><doi>10.1002/pmic.200401133</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1615-9853 |
| ispartof | Proteomics (Weinheim), 2005-08, Vol.5 (12), p.3173-3182 |
| issn | 1615-9853 1615-9861 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68464986 |
| source | Wiley |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biomarkers, Tumor - chemistry Blotting, Western Carcinoma - metabolism Cell Line, Tumor Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Galectin 3 - metabolism Humans Immunohistochemistry Mass Spectrometry Medical sciences Mice Mice, Inbred BALB C Mice, Nude Miscellaneous Nasopharyngeal carcinoma Nasopharyngeal Neoplasms - metabolism Neoplasm Transplantation Otorhinolaryngology. Stomatology Plasminogen Activator Inhibitor 1 - metabolism Proteins Proteomics - methods Secreted proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor markers Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Cancer cell-secreted proteomes as a basis for searching potential tumor markers: Nasopharyngeal carcinoma as a model |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T00%3A33%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cancer%20cell-secreted%20proteomes%20as%20a%20basis%20for%20searching%20potential%20tumor%20markers:%20Nasopharyngeal%20carcinoma%20as%20a%20model&rft.jtitle=Proteomics%20(Weinheim)&rft.au=Wu,%20Chih-Ching&rft.date=2005-08-01&rft.volume=5&rft.issue=12&rft.spage=3173&rft.epage=3182&rft.pages=3173-3182&rft.issn=1615-9853&rft.eissn=1615-9861&rft_id=info:doi/10.1002/pmic.200401133&rft_dat=%3Cproquest_cross%3E68464986%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4423-898e8bea2c2e340ea00e08c90b36cebcea4006557cefc0f453e4ee7d2ed554c93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17593484&rft_id=info:pmid/16035111&rfr_iscdi=true |