Mammalian SEPT2 Is Required for Scaffolding Nonmuscle Myosin II and Its Kinases

Mammalian septin SEPT2 belongs to a conserved family of filamentous GTPases that are associated with actin stress fibers in interphase cells and the contractile ring in dividing cells. Although SEPT2 is essential for cytokinesis, its role in this process remains undefined. Here, we report that SEPT2...

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Bibliographic Details
Published in:Developmental cell 2007-11, Vol.13 (5), p.677-690
Main Authors: Joo, Emily, Surka, Mark C., Trimble, William S.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
CELLBIO
CHO Cells
Cricetinae
Cricetulus
Cytokinesis
Fundamental and applied biological sciences. Psychology
GTP Phosphohydrolases - physiology
Intracellular Signaling Peptides and Proteins - metabolism
Molecular and cellular biology
Myosin Type II - metabolism
Protein Binding
Protein Folding
Protein-Serine-Threonine Kinases - metabolism
rho-Associated Kinases - metabolism
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Summary:Mammalian septin SEPT2 belongs to a conserved family of filamentous GTPases that are associated with actin stress fibers in interphase cells and the contractile ring in dividing cells. Although SEPT2 is essential for cytokinesis, its role in this process remains undefined. Here, we report that SEPT2 directly binds nonmuscle myosin II (myosin II), and this association is important for fully activating myosin II in interphase and dividing cells. Inhibition of the SEPT2-myosin II interaction in interphase cells results in loss of stress fibers, while in dividing cells this causes instability of the ingressed cleavage furrow and dissociation of the myosin II from the Rho-activated myosin kinases ROCK and citron kinase. We propose that SEPT2-containing filaments provide a molecular platform for myosin II and its kinases to ensure the full activation of myosin II that is necessary for the final stages of cytokinesis.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2007.09.001