A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma

A novel strategy consisting of cleavable Isotope‐Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in ‘abnormal’ patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-08, Vol.5 (12), p.3040-3044
Main Authors: Leung, Kit-Yi, Lescuyer, Pierre, Campbell, James, Byers, Helen L., Allard, Laure, Sanchez, Jean-Charles, Ward, Malcolm A.
Format: Article
Language:English
Subjects:
Algorithms
Animals
Biomarkers
Blood Proteins - chemistry
Cleavable isotope-coded affinity tag
Comparative proteomics
Computational Biology - methods
Databases, Protein
Humans
Immunoglobulin G - chemistry
Ions
Isotopes
MACCOT Distiller
Mass Spectrometry - methods
Peptide Mapping
Peptides - chemistry
Plasma
Proteins - chemistry
Proteome
Proteomics - methods
Software
Time Factors
Up-Regulation
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Summary:A novel strategy consisting of cleavable Isotope‐Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in ‘abnormal’ patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT‐labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200402101