Identification of genes associated with tumorigenesis of meibomian cell carcinoma by microarray analysis

Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identi...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2007-11, Vol.90 (5), p.559-566
Main Authors: Kumar, Arun, Kumar Dorairaj, Syril, Prabhakaran, Venkatesh C., Prakash, D. Ravi, Chakraborty, Sanjukta
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Case-Control Studies
Cell physiology
Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes
Expression profile
Eyelid Neoplasms - genetics
Eyelid Neoplasms - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes. Genome
Genetics of eukaryotes. Biological and molecular evolution
Humans
JAK/STAT pathway
MAPK signaling pathway
Meibomian cell carcinoma
Meibomian Glands - pathology
Microarrays
Middle Aged
Molecular and cellular biology
Molecular genetics
Neoplasm Proteins - genetics
Oligonucleotide Array Sequence Analysis - methods
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal Transduction
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Summary:Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identify genes and pathways that are involved in the development and progression of MCC. We analyzed the gene expression profile of MCC by the microarray technique. Forty-four genes were upregulated and 149 genes were downregulated in MCC. Differential expression data were confirmed for 5 genes by semiquantitative RT-PCR in MCC tumors: GTF2H4, RBM12, UBE2D3, DDX17, and LZTS1. We found dysregulation of two major pathways in MCC: MAPK and JAK/STAT. Clusters of genes on chromosomes 1, 12, and 19 were dysregulated in MCC. The data presented here will facilitate the identification of specific markers and therapeutic targets for the treatment of MCC patients.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2007.07.008