Cellular fate of oligonucleotides when delivered by nanocapsules of poly(isobutylcyanoacrylate)

Dual-labeled phosphorothioate oligonucleotides were encapsulated in the internal aqueous core of poly(isobutylcyanoacrylate) nanocapsules with high yields. Intracellular distribution of naked and encapsulated oligomers was assessed on cultured vascular smooth muscle cells using cell fractionation an...

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Bibliographic Details
Published in:Journal of controlled release 2005-08, Vol.106 (1), p.209-213
Main Authors: Toub, Nedjma, Angiari, Chiara, Eboué, Dominique, Fattal, Elias, Tenu, Jean-Pierre, Le Doan, Trung, Couvreur, Patrick
Format: Article
Language:English
Subjects:
Animals
Aorta, Thoracic
Biodistribution
Biological and medical sciences
Capsules
Cell fractionation
Cells, Cultured
Cyanoacrylates
Drug Carriers
General pharmacology
Medical sciences
Microscopy, Confocal
Muscle, Smooth, Vascular - metabolism
Myocytes, Smooth Muscle - metabolism
Nanocapsules
Nanotechnology
Oligonucleotides
Oligonucleotides - metabolism
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polymers
Rats
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Summary:Dual-labeled phosphorothioate oligonucleotides were encapsulated in the internal aqueous core of poly(isobutylcyanoacrylate) nanocapsules with high yields. Intracellular distribution of naked and encapsulated oligomers was assessed on cultured vascular smooth muscle cells using cell fractionation and confocal microscopy methods. The results showed that cell uptake and more interestingly, nuclear accumulation of oligonucleotides were importantly enhanced when the oligonucleotides were associated to the nanocapsules as compared to the naked oligomers. These results suggested an efficient capacity of this polymeric carrier to deliver its payload to the cytosol and nucleus of smooth muscle cells.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2005.03.029