Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH)

X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in...

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Bibliographic Details
Published in:Cell biology international 2005-08, Vol.29 (8), p.647-653
Main Authors: Zambelli, A., Mongiardini, E., Villegas, S.N., Carri, N.G., Boot-Handford, R.P., Wallis, G.A.
Format: Article
Language:English
Subjects:
Animals
Ascorbic Acid - pharmacology
Blotting, Northern
Cell Differentiation - drug effects
Cell Nucleus - metabolism
Cytoplasm - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Fluorescent Antibody Technique
Gene Expression Regulation - drug effects
Glycerophosphates - pharmacology
Mice
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Osteoblast differentiation
Osteoblasts - cytology
Osteoblasts - metabolism
Parathyroid Hormone - pharmacology
PTH
Regulatory Factor X Transcription Factors
RNA, Messenger - analysis
Trans-Activators
Transcription Factors
X-Box Binding Protein 1
XBP-1
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Summary:X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and β-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter.
ISSN:1065-6995
1095-8355
DOI:10.1016/j.cellbi.2005.03.018