An indirect immunocolorimetric assay to detect rubella virus infected cells

An indirect immunocolorimetric assay (ICA) to detect rubella virus infected cells by the naked eye was developed. This assay was as sensitive and specific as an indirect immunofluorescent assay (IFA), could detect as little as 10 plaque forming units (pfu) of rubella virus in the initial inoculum an...

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Bibliographic Details
Published in:Journal of virological methods 2007-12, Vol.146 (1), p.414-418
Main Authors: Chen, Min-hsin, Zhu, Zhen, Zhang, Yan, Favors, Sheena, Xu, Wen-bo, Featherstone, David A., Icenogle, Joseph P.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Line
Detection of rubella virus
Fundamental and applied biological sciences. Psychology
Humans
Immunoassay - methods
Indirect immunocolorimetric assay
Measles - virology
Measles virus
Measles virus - immunology
Measles virus - isolation & purification
Microbiology
Noncytopathic
Pharynx - virology
Rubella - diagnosis
Rubella - virology
Rubella virus
Rubella virus - immunology
Rubella virus - isolation & purification
Sensitivity and Specificity
Techniques used in virology
Virology
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Summary:An indirect immunocolorimetric assay (ICA) to detect rubella virus infected cells by the naked eye was developed. This assay was as sensitive and specific as an indirect immunofluorescent assay (IFA), could detect as little as 10 plaque forming units (pfu) of rubella virus in the initial inoculum and could detect viruses from throat swabs. This assay detected infection with viruses of all nine rubella virus genotypes available for testing. It could be utilized for virus quantitation and a neutralization assay. In addition to detecting rubella virus infection, this assay also allowed us to confirm measles virus infection. This assay should be useful for virus isolation from clinical samples.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2007.08.021