Determination of gastric emptying in nonobese diabetic mice

Animal studies on diabetic gastroparesis are limited by inability to follow gastric emptying changes in the same mouse. The study aim was to validate a nonlethal gastric emptying method in nonobese diabetic (NOD) LtJ mice, a model of type 1 diabetes, and study sequential changes with age and early d...

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Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 2007-11, Vol.293 (5), p.G1039-G1045
Main Authors: Choi, Kyoung Moo, Zhu, Jin, Stoltz, Gary J, Vernino, Steven, Camilleri, Michael, Szurszewski, Joseph H, Gibbons, Simon J, Farrugia, Gianrico
Format: Article
Language:English
Subjects:
Acids
Aging
Aging - physiology
Animals
Breath Tests
Carbon Isotopes
Diabetes
Diabetes Mellitus, Type 1 - physiopathology
Disease Models, Animal
Female
Gastric Emptying - physiology
Kinetics
Mice
Mice, Inbred NOD
Rodents
Stomach
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Summary:Animal studies on diabetic gastroparesis are limited by inability to follow gastric emptying changes in the same mouse. The study aim was to validate a nonlethal gastric emptying method in nonobese diabetic (NOD) LtJ mice, a model of type 1 diabetes, and study sequential changes with age and early diabetic status. The reliability and responsiveness of a [(13)C]octanoic acid breath test in NOD LtJ mice was tested, and the test was used to measure solid gastric emptying in NOD LtJ mice and nonobese diabetes resistant (NOR) LtJ mice. The (13)C breath test produced results similar to postmortem recovery of a meal. Bethanechol accelerated gastric emptying [control: 92 +/- 9 min; bethanechol: 53 +/- 3 min, mean half emptying time (T(1/2)) +/- SE], and atropine slowed gastric emptying (control: 92 +/- 9 min; atropine: 184 +/- 31 min, mean T(1/2) +/- SE). Normal gastric emptying (T(1/2)) in nondiabetic NOD LtJ mice (8-12 wk) was 91 +/- 2 min. Aging had differing effects on gastric emptying in NOD LtJ and NOR LtJ mice. Onset of diabetes was accompanied by accelerated gastric emptying during weeks 1-2 of diabetes. Gastric emptying returned to normal by weeks 3-5 with no delay. The [(13)C]octanoic acid breath test accurately measures gastric emptying in NOD LtJ mice, is useful to study the time course of changes in gastric emptying in diabetic NOD LtJ mice, and is able to detect acceleration in gastric emptying early in diabetes. Opposing changes in gastric emptying between NOD LtJ and NOR LtJ mice suggest that NOR LtJ mice are not good controls for the study of gastric emptying in NOD LtJ mice.
ISSN:0193-1857
1522-1547
DOI:10.1152/ajpgi.00317.2007