Pre- and post-exposure protection against Western equine encephalitis virus after single inoculation with adenovirus vector expressing interferon alpha

Abstract Western equine encephalitis virus ( WEEV ) is a positive-sense, single-stranded RNA virus which is transmitted to equines and humans through mosquito bites. WEEV infects the central nervous system with severe complications and even death. There are no human vaccine and antiviral drugs. We i...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2007-12, Vol.369 (1), p.206-213
Main Authors: Wu, Josh Q.H, Barabé, Nicole D, Huang, Ying-Min, Rayner, George A, Christopher, Mary E, Schmaltz, Fay L
Format: Article
Language:English
Subjects:
Adenoviridae
Adenoviridae - genetics
Adenovirus
Adenovirus vector
Alphavirus
Animals
disease control
Encephalitis Virus, Western Equine - immunology
Encephalomyelitis, Equine - immunology
Encephalomyelitis, Equine - prevention & control
Female
Genetic Therapy - methods
Genetic Vectors
human health
Immunotherapy - methods
Infectious Disease
Interferon alpha
Interferon-alpha - biosynthesis
Interferon-alpha - genetics
Interferon-alpha - immunology
interferons
Mice
Mice, Inbred BALB C
Survival Analysis
transgenes
Western equine encephalitis virus
Western equine encephalomyelitis
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Summary:Abstract Western equine encephalitis virus ( WEEV ) is a positive-sense, single-stranded RNA virus which is transmitted to equines and humans through mosquito bites. WEEV infects the central nervous system with severe complications and even death. There are no human vaccine and antiviral drugs. We investigated whether adenovirus-mediated expression of interferon alpha could be used for pre- and post-exposure protection against a lethal WEEV challenge in mice. A human adenoviral vector (Ad5-mIFNα) expressing mouse interferon alpha was constructed. We found that Ad5-mIFNα provided 100% protection against various WEEV strains in mice after a single intramuscular inoculation at 24 h, 48 h or 1 week before the challenge. When given as a single inoculation at 6 h after the challenge, Ad5-mIFNα delayed the progress of WEEV infection and provided about 60% protection. Our findings suggest that adenovirus-mediated expression of interferon alpha can be an alternative approach for the prevention and treatment of WEEV infection.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.07.024