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Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells
Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D 2 O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells. Material...
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| Published in: | Anticancer research 2005-09, Vol.25 (5), p.3407-3411 |
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| Main Authors: | , , , , , , , , , , |
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| container_end_page | 3411 |
| container_issue | 5 |
| container_start_page | 3407 |
| container_title | Anticancer research |
| container_volume | 25 |
| creator | HARTMANN, Johannes BADER, Yvonne SZEKERES, Thomas HORVATH, Zsuzsanna SAIKO, Philipp GRUSCH, Michael ILLMER, Christoph MADLENER, Sibylle FRITZER-SZEKERES, Monika HELLER, Nicole ALKEN, Rudolf-Giesbert |
| description | Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy
water (deuterium oxide, D 2 O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic
tumor cells. Materials and Methods: The cytotoxic effects of D 2 O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by
Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing
a clonogenic assay, D 2 O yielded IC 50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared
to untreated controls. Moreover, D 2 O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D 2 O might offer an additional option for the treatment of pancreatic carcinomas. |
| format | article |
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water (deuterium oxide, D 2 O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic
tumor cells. Materials and Methods: The cytotoxic effects of D 2 O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by
Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing
a clonogenic assay, D 2 O yielded IC 50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared
to untreated controls. Moreover, D 2 O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D 2 O might offer an additional option for the treatment of pancreatic carcinomas.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 16101156</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Adenocarcinoma - drug therapy ; Adenocarcinoma - pathology ; Antineoplastic Agents - pharmacology ; Apoptosis - drug effects ; Biological and medical sciences ; Cell Cycle - drug effects ; Cell Growth Processes - drug effects ; Cell Line, Tumor ; Deuterium Oxide - pharmacology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Medical sciences ; Pancreatic Neoplasms - drug therapy ; Pancreatic Neoplasms - pathology ; Tumors</subject><ispartof>Anticancer research, 2005-09, Vol.25 (5), p.3407-3411</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16992758$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16101156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HARTMANN, Johannes</creatorcontrib><creatorcontrib>BADER, Yvonne</creatorcontrib><creatorcontrib>SZEKERES, Thomas</creatorcontrib><creatorcontrib>HORVATH, Zsuzsanna</creatorcontrib><creatorcontrib>SAIKO, Philipp</creatorcontrib><creatorcontrib>GRUSCH, Michael</creatorcontrib><creatorcontrib>ILLMER, Christoph</creatorcontrib><creatorcontrib>MADLENER, Sibylle</creatorcontrib><creatorcontrib>FRITZER-SZEKERES, Monika</creatorcontrib><creatorcontrib>HELLER, Nicole</creatorcontrib><creatorcontrib>ALKEN, Rudolf-Giesbert</creatorcontrib><title>Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy
water (deuterium oxide, D 2 O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic
tumor cells. Materials and Methods: The cytotoxic effects of D 2 O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by
Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing
a clonogenic assay, D 2 O yielded IC 50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared
to untreated controls. Moreover, D 2 O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D 2 O might offer an additional option for the treatment of pancreatic carcinomas.</description><subject>Adenocarcinoma - drug therapy</subject><subject>Adenocarcinoma - pathology</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Growth Processes - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Deuterium Oxide - pharmacology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Medical sciences</subject><subject>Pancreatic Neoplasms - drug therapy</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpNz81Kw0AUBeBBFFurryCzUXQRuJPJTBJwI7VaoVAXFZfD7eSOjeSnziRK396IFV2dzcfhnAM2FmkuolRJOGRjiBVEKYAasZMQ3gC0zjN5zEZCCxBC6TG7mTlHtgu8dXxO-LHjL9iR51d38fKatw2f9zU2_Akb6wm70vJVX7eeT6mqwik7clgFOtvnhD3fz1bTebRYPjxObxfRJtZ5F2l0VsWYZYVABTmA1YVNBBUAEmSc0DpzKCwRWg0KlFTaSUrIJnJNKEBO2OVP79a37z2FztRlsMMCbKjtg9FZkgmhv-H5Hvbrmgqz9WWNfmd-_w7gYg8wWKycH36V4Z_L8zhV2Z_blK-bz9KTCTVW1VArDfpYGWVkAqn8Aqw1aZ0</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>HARTMANN, Johannes</creator><creator>BADER, Yvonne</creator><creator>SZEKERES, Thomas</creator><creator>HORVATH, Zsuzsanna</creator><creator>SAIKO, Philipp</creator><creator>GRUSCH, Michael</creator><creator>ILLMER, Christoph</creator><creator>MADLENER, Sibylle</creator><creator>FRITZER-SZEKERES, Monika</creator><creator>HELLER, Nicole</creator><creator>ALKEN, Rudolf-Giesbert</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells</title><author>HARTMANN, Johannes ; BADER, Yvonne ; SZEKERES, Thomas ; HORVATH, Zsuzsanna ; SAIKO, Philipp ; GRUSCH, Michael ; ILLMER, Christoph ; MADLENER, Sibylle ; FRITZER-SZEKERES, Monika ; HELLER, Nicole ; ALKEN, Rudolf-Giesbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-6afc52a88d1a50900c6dc41ed0030324eb8fa1ceeac60505356f3e4ec43bea103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenocarcinoma - drug therapy</topic><topic>Adenocarcinoma - pathology</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Growth Processes - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Deuterium Oxide - pharmacology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Medical sciences</topic><topic>Pancreatic Neoplasms - drug therapy</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HARTMANN, Johannes</creatorcontrib><creatorcontrib>BADER, Yvonne</creatorcontrib><creatorcontrib>SZEKERES, Thomas</creatorcontrib><creatorcontrib>HORVATH, Zsuzsanna</creatorcontrib><creatorcontrib>SAIKO, Philipp</creatorcontrib><creatorcontrib>GRUSCH, Michael</creatorcontrib><creatorcontrib>ILLMER, Christoph</creatorcontrib><creatorcontrib>MADLENER, Sibylle</creatorcontrib><creatorcontrib>FRITZER-SZEKERES, Monika</creatorcontrib><creatorcontrib>HELLER, Nicole</creatorcontrib><creatorcontrib>ALKEN, Rudolf-Giesbert</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HARTMANN, Johannes</au><au>BADER, Yvonne</au><au>SZEKERES, Thomas</au><au>HORVATH, Zsuzsanna</au><au>SAIKO, Philipp</au><au>GRUSCH, Michael</au><au>ILLMER, Christoph</au><au>MADLENER, Sibylle</au><au>FRITZER-SZEKERES, Monika</au><au>HELLER, Nicole</au><au>ALKEN, Rudolf-Giesbert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>25</volume><issue>5</issue><spage>3407</spage><epage>3411</epage><pages>3407-3411</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy
water (deuterium oxide, D 2 O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic
tumor cells. Materials and Methods: The cytotoxic effects of D 2 O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by
Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing
a clonogenic assay, D 2 O yielded IC 50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared
to untreated controls. Moreover, D 2 O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D 2 O might offer an additional option for the treatment of pancreatic carcinomas.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>16101156</pmid><tpages>5</tpages></addata></record> |
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| language | eng |
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| subjects | Adenocarcinoma - drug therapy Adenocarcinoma - pathology Antineoplastic Agents - pharmacology Apoptosis - drug effects Biological and medical sciences Cell Cycle - drug effects Cell Growth Processes - drug effects Cell Line, Tumor Deuterium Oxide - pharmacology Gastroenterology. Liver. Pancreas. Abdomen Humans Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Pancreatic Neoplasms - drug therapy Pancreatic Neoplasms - pathology Tumors |
| title | Effects of Heavy Water (D2O) on Human Pancreatic Tumor Cells |
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