In vitro and in vivo optimization of impaction allografting by demineralization and addition of rh-OP-1

Impaction allografting is a bone tissue engineering technique currently used in lower limb reconstruction orthopedic surgery. Our hypothesis was that biological optimization can be achieved by demineralization and addition of osteogenic protein‐1 (OP‐1) to the allograft. The objective of our in vitr...

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Bibliographic Details
Published in:Journal of orthopaedic research 2007-11, Vol.25 (11), p.1425-1437
Main Authors: Tsiridis, Eleftherios, Ali, Zubier, Bhalla, Amit, Heliotis, Manolis, Gurav, Neelam, Deb, Sanjukta, DiSilvio, Lucy
Format: Article
Language:English
Subjects:
Adult
Alkaline Phosphatase - metabolism
allografting
Animals
arthroplasty
Bone Demineralization Technique
Bone Marrow Cells - drug effects
Bone Marrow Cells - ultrastructure
Bone Matrix - transplantation
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins - pharmacology
Bone Transplantation - instrumentation
Bone Transplantation - methods
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Cells, Cultured
demineralization
hip
Humans
impaction
Male
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - enzymology
Mesenchymal Stromal Cells - ultrastructure
OP-1
Osteogenesis - drug effects
Osteogenesis - physiology
Rats
Rats, Wistar
revision
Tissue Engineering - instrumentation
Tissue Engineering - methods
Transplantation, Homologous
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Summary:Impaction allografting is a bone tissue engineering technique currently used in lower limb reconstruction orthopedic surgery. Our hypothesis was that biological optimization can be achieved by demineralization and addition of osteogenic protein‐1 (OP‐1) to the allograft. The objective of our in vitro study was to evaluate human mesenchymal stem cell (MSC) proliferation (Alamar Blue assay, titrated thymidine assay, total DNA Hoechst 33258, and scanning electron microscopy) and osteogenic differentiation (alkaline phosphatase assay) in two types of impacted carrier, namely, demineralized bone matrix (DBM) and insoluble collagenous bone matrix (ICBM), with or without OP‐1. The objective in vivo was to compare the osteogenic potential of impacted DBM with or without OP‐1, with that of impacted fresh frozen allograft (FFA), again with or without OP‐1. DBM + OP‐1 optimized osteoinduction and significantly improved (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.20387