Reactivation of Antigen‐Induced Arthritis in Mice by Oral Administration of Lipopolysaccharide

We examined whether oral administration of lipopolysaccharide (LPS) from Escherichia coli reactivated antigen‐induced arthritis (AIA) in mice that is one of models of human rheumatoid arthritis. To induce AIA, mice were immunized by subcutaneous injection of ovalbumin (OVA) emulsified with complete...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of immunology 2005-08, Vol.62 (2), p.117-122
Main Authors: Yoshino, S., Yamaki, K., Taneda, S., Yanagisawa, R., Takano, H.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Arthritis, Experimental - immunology
Arthritis, Experimental - pathology
Arthritis, Rheumatoid - immunology
Arthritis, Rheumatoid - pathology
Disease Models, Animal
Escherichia coli
Hindlimb - immunology
Hindlimb - pathology
Immunoglobulin G - blood
Interferon-gamma - immunology
Interleukin-4 - immunology
Joints - immunology
Joints - pathology
Lipopolysaccharides - immunology
Male
Mice
Mice, Inbred DBA
Ovalbumin - immunology
Polymyxin B - pharmacology
Spleen - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examined whether oral administration of lipopolysaccharide (LPS) from Escherichia coli reactivated antigen‐induced arthritis (AIA) in mice that is one of models of human rheumatoid arthritis. To induce AIA, mice were immunized by subcutaneous injection of ovalbumin (OVA) emulsified with complete Freund's adjuvant into the base of the tail (day 0) followed by intraarticular injection of OVA on day 21. To investigate the ability of LPS to reactivate AIA, varying doses of LPS were p.o. administered 48 h after the challenge injection. The results showed that administration of LPS was followed by reactivation of AIA in a dose‐related fashion. The reactivation of AIA by LPS was associated with increases in interferon‐γ, interleukin‐1β and tumour necrosis factor‐α. Polymyxin B sulfate given immediately before administration of LPS suppressed the reactivation of AIA. These findings suggest that LPS from intestinal bacteria may play a role in the reactivation of joint inflammation in which immune responses to pathogenic antigens are involved.
ISSN:0300-9475
1365-3083
DOI:10.1111/j.1365-3083.2005.01647.x