Sex differences in cytokine production and surface antigen expression of peripheral blood mononuclear cells after surgery

• Published in: The American journal of surgery 2005-09, Vol.190 (3), p.439-444
• Main Authors: Ono, Satoshi, Tsujimoto, Hironori, Hiraki, Shu-ichi, Takahata, Risa, Kinoshita, Manabu, Mochizuki, Hidetaka
• Format: Article
• Language: English
• Subjects: Adult; Antigens, Surface - metabolism; Biological and medical sciences; Blood; CD14+ CD16+ monocytes; Cytokines; Cytokines - metabolism; Disease Susceptibility; Female; Gastrectomy - adverse effects; Gender differences; General aspects; HLA-DR Antigens - blood; Human leukocyte antigen–D related; Humans; Immune system; Immunology; Interferon-gamma - blood; Interferon-γ; Interleukin 10; Interleukin-10 - blood; Japan - epidemiology; Leukocytes, Mononuclear - metabolism; Male; Medical sciences; Membrane Glycoproteins - blood; Middle Aged; Postoperative Complications - epidemiology; Postoperative Complications - immunology; Receptors, Cell Surface - blood; Receptors, IgG - blood; Sex Factors; Stomach Neoplasms - immunology; Stomach Neoplasms - surgery; Stress, Physiological - etiology; Stress, Physiological - immunology; Systemic Inflammatory Response Syndrome - epidemiology; Systemic Inflammatory Response Syndrome - immunology; Toll-like receptor; Toll-Like Receptor 2; Toll-Like Receptor 4; Toll-Like Receptors; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α
• ISSN: 0002-9610; 1879-1883
• DOI: 10.1016/j.amjsurg.2005.03.031

Several clinical and epidemiological studies have observed a better outcome after sepsis in women than in men. The purpose of this study was to determine if these sex differences are observed in cytokine responses and the surface antigen expression of monocytes. In addition, the clinical courses of male and female patients after gastrointestinal surgery were compared. A total of 25 patients with gastric carcinoma who underwent gastrectomy were enrolled in this study. Tumor necrosis factor-α (TNF-α), interleukin-10, and interferon-γ (IFN-γ) production by lipopolysaccharide-stimulated peripheral blood mononuclear cells (PBMCs) as well as the expression of Toll-like receptor-4 (TLR-4), TLR-2, human leukocyte antigen–D related (HLA-DR), and CD16 on monocytes in 16 men and 9 women on the day before surgery were compared with measurements on postoperative day (POD) 1. Furthermore, postoperative infectious complications, the development of systemic inflammatory response syndrome, and serum C-reactive protein levels on POD3 were compared. TNF-α production of PBMCs and TLR-2 and CD16 expression on monocytes were significantly higher in women than in men before surgery. IFN-γ production of PBMCs and HLA-DR expression on monocytes were significantly lower in men than in women on POD1. Furthermore, TNF-α production of PBMCs on POD1 was significantly increased, and both IFN-γ production and HLA-DR expression were significantly decreased compared with that observed before surgery in men, but no corresponding significant changes were observed in women. In addition, C-reactive protein levels on POD3 were significantly higher in men than in women. Both TNF-α and interleukin-10 production of PBMCs and both TLR-2 and CD16 expression on monocytes were significantly higher in women than in men on the day before surgery. Excessive TNF-α and suppressive IFN-γ production of PBMCs, as well as a decrease in HLA-DR expression on monocytes, occurred more often in men than in women after surgery, suggesting that these factors all contribute to an increased susceptibility of men to develop systemic inflammatory response syndrome or postoperative infectious complications.