Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface

Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separ...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2007-11, Vol.60 (2), p.264-267
Main Authors: Wang, Peng, Li, Hai, Yang, Hai Jun, Wang, Hua Bing, Lü, Jun Hong, Zhang, Yi, Hu, Jun
Format: Article
Language:English
Subjects:
Adeno-associated virus
Aluminum Silicates - chemistry
Atomic force microscope
Dependovirus - chemistry
Dependovirus - classification
Disaggregation
Dynamic light scattering
Glycerol
Glycerol - chemistry
Microscopy, Atomic Force - methods
Particle Size
Protein Binding
Recombinant adeno-associated virus 2 (rAAV2)
Recombinant Proteins - chemistry
Recombinant Proteins - classification
Surface Properties
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Summary:Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2007.06.027