Demonstration of a Glycoprotein Derived From the Ceacam10 Gene in Mouse Seminal Vesicle Secretions

CEACAM10 was purified from mouse seminal vesicle secretions by a series of purification steps that included ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was shown to be a 36-kDa glycoprotein with an N-linked...

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Bibliographic Details
Published in:Biology of reproduction 2005-09, Vol.73 (3), p.546-553
Main Authors: Li, Sheng-Hsiang, Lee, Robert Kuo-Kuang, Hsiao, Ya-Ling, Chen, Yee-Hsiung
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cell Adhesion Molecules - chemistry
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - isolation & purification
Cell Adhesion Molecules - physiology
Epithelial Cells - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Male
Male Reproductive Tract
Mammalian male genital system
Mice
Molecular Sequence Data
Morphology. Physiology
RNA, Messenger
Seminal Vesicles - cytology
Seminal Vesicles - metabolism
Sperm Motility - physiology
Spermatozoa - physiology
Vertebrates: reproduction
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Summary:CEACAM10 was purified from mouse seminal vesicle secretions by a series of purification steps that included ion exchange chromatography on a DEAE-Sephacel column and ion exchange high-performance liquid chromatography on a sulfopropyl column. It was shown to be a 36-kDa glycoprotein with an N-linked carbohydrate moiety. The circular dichromoism spectrum of CEACAM10 in 50 mM phosphate buffer at pH 7.4 appeared as one negative band arising from the {szligbeta} form at 217 nm. CEACAM10 was expressed predominantly in seminal vesicles of adult mice. Both CEACAM10 and its mRNA were demonstrated on the luminal epithelium of the mucosal folds in the seminal vesicle. The amount of Ceacam10 mRNA in the seminal vesicle was correlated with the stage of animal maturation. Castration of adult mice resulted in cessation of Ceacam10 expression, while treatment of castrated mice with testosterone propionate in corn oil restored Ceacam10 expression in the seminal vesicle. During the entire course of pregnancy, Ceacam10 might be silent in the embryo. A cytochemical study illustrated the presence of the CEACAM10 binding region on the entire surface of mouse sperm. CEACAM10-sperm binding greatly enhanced sperm motility in vitro.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.105.039651