A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium

A sucrose-inducible promoter system (P sacB ) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l −1) and the heterologous green fluorescent protein GFP (7.9 mg g CDW −1). Mu...

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Bibliographic Details
Published in:Journal of biotechnology 2007-12, Vol.132 (4), p.426-430
Main Authors: Biedendieck, Rebekka, Gamer, Martin, Jaensch, Lothar, Meyer, Steffen, Rohde, Manfred, Deckwer, Wolf-Dieter, Jahn, Dieter
Format: Article
Language:English
Subjects:
Bacillus megaterium
Bacillus megaterium - metabolism
DNA Mutational Analysis
Green Fluorescent Proteins - metabolism
Hexosyltransferases - metabolism
Promoter Regions, Genetic
Recombinant protein production
Recombinant Proteins - metabolism
Sucrose - metabolism
Sucrose-inducible promoter
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Summary:A sucrose-inducible promoter system (P sacB ) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l −1) and the heterologous green fluorescent protein GFP (7.9 mg g CDW −1). Mutational analysis of B. megaterium P sacB allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P xylA ) for recombinant gene expression in B. megaterium.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2007.07.494