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A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium
A sucrose-inducible promoter system (P sacB ) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U l −1) and the heterologous green fluorescent protein GFP (7.9 mg g CDW −1). Mu...
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| Published in: | Journal of biotechnology 2007-12, Vol.132 (4), p.426-430 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c394t-a6fd78901c1c5717d15e0aea9fd28b2d8241b55925166388d9c81d034bc4b0403 |
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| cites | cdi_FETCH-LOGICAL-c394t-a6fd78901c1c5717d15e0aea9fd28b2d8241b55925166388d9c81d034bc4b0403 |
| container_end_page | 430 |
| container_issue | 4 |
| container_start_page | 426 |
| container_title | Journal of biotechnology |
| container_volume | 132 |
| creator | Biedendieck, Rebekka Gamer, Martin Jaensch, Lothar Meyer, Steffen Rohde, Manfred Deckwer, Wolf-Dieter Jahn, Dieter |
| description | A sucrose-inducible promoter system (P
sacB
) from
Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4
U
l
−1) and the heterologous green fluorescent protein GFP (7.9
mg
g
CDW
−1). Mutational analysis of
B. megaterium P
sacB
allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P
xylA
) for recombinant gene expression in
B. megaterium. |
| doi_str_mv | 10.1016/j.jbiotec.2007.07.494 |
| format | article |
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sacB
) from
Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4
U
l
−1) and the heterologous green fluorescent protein GFP (7.9
mg
g
CDW
−1). Mutational analysis of
B. megaterium P
sacB
allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P
xylA
) for recombinant gene expression in
B. megaterium.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2007.07.494</identifier><identifier>PMID: 17692983</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacillus megaterium ; Bacillus megaterium - metabolism ; DNA Mutational Analysis ; Green Fluorescent Proteins - metabolism ; Hexosyltransferases - metabolism ; Promoter Regions, Genetic ; Recombinant protein production ; Recombinant Proteins - metabolism ; Sucrose - metabolism ; Sucrose-inducible promoter</subject><ispartof>Journal of biotechnology, 2007-12, Vol.132 (4), p.426-430</ispartof><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-a6fd78901c1c5717d15e0aea9fd28b2d8241b55925166388d9c81d034bc4b0403</citedby><cites>FETCH-LOGICAL-c394t-a6fd78901c1c5717d15e0aea9fd28b2d8241b55925166388d9c81d034bc4b0403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17692983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biedendieck, Rebekka</creatorcontrib><creatorcontrib>Gamer, Martin</creatorcontrib><creatorcontrib>Jaensch, Lothar</creatorcontrib><creatorcontrib>Meyer, Steffen</creatorcontrib><creatorcontrib>Rohde, Manfred</creatorcontrib><creatorcontrib>Deckwer, Wolf-Dieter</creatorcontrib><creatorcontrib>Jahn, Dieter</creatorcontrib><title>A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>A sucrose-inducible promoter system (P
sacB
) from
Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4
U
l
−1) and the heterologous green fluorescent protein GFP (7.9
mg
g
CDW
−1). Mutational analysis of
B. megaterium P
sacB
allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P
xylA
) for recombinant gene expression in
B. megaterium.</description><subject>Bacillus megaterium</subject><subject>Bacillus megaterium - metabolism</subject><subject>DNA Mutational Analysis</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hexosyltransferases - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant protein production</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sucrose - metabolism</subject><subject>Sucrose-inducible promoter</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkU1LJDEQhoO4OOPHT1By8tazSeejk5Oo7OqCsJfdc0gn1ZqhP8YkLfrvTTMDHoWCqsPzvlXUi9AlJRtKqPy53WzbMGVwm5qQZlOKa36E1lQ1rOJKsmO0LpyqqBRyhU5T2hJCuBb0BK1oI3WtFVuj8Ran2cUpQRVGP7vQ9oB3cRqKdcTpI2UYcDdFnF8AhzFHW2E7egzvZXTQ93Nv4yLIEMalF48cprGw-M66UICEB3i2xS7Mwzn60dk-wcWhn6H_v3_9u3-snv4-_Lm_faoc0zxXVna-UZpQR51oaOOpAGLB6s7Xqq29qjlthdC1oFIypbx2inrCeOt4SzhhZ-h671suep0hZTOEtJxrR5jmZKTimqnme5DqhikhFlDsweVZKUJndjEMNn4YSsySiNmaQyJmScSUKokU3dVhwdwO4L9UhwgKcLMHoPzjLUA0yQUYHfgQwWXjp_DNik8Zn6ED</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Biedendieck, Rebekka</creator><creator>Gamer, Martin</creator><creator>Jaensch, Lothar</creator><creator>Meyer, Steffen</creator><creator>Rohde, Manfred</creator><creator>Deckwer, Wolf-Dieter</creator><creator>Jahn, Dieter</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium</title><author>Biedendieck, Rebekka ; Gamer, Martin ; Jaensch, Lothar ; Meyer, Steffen ; Rohde, Manfred ; Deckwer, Wolf-Dieter ; Jahn, Dieter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-a6fd78901c1c5717d15e0aea9fd28b2d8241b55925166388d9c81d034bc4b0403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacillus megaterium</topic><topic>Bacillus megaterium - metabolism</topic><topic>DNA Mutational Analysis</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Hexosyltransferases - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant protein production</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sucrose - metabolism</topic><topic>Sucrose-inducible promoter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biedendieck, Rebekka</creatorcontrib><creatorcontrib>Gamer, Martin</creatorcontrib><creatorcontrib>Jaensch, Lothar</creatorcontrib><creatorcontrib>Meyer, Steffen</creatorcontrib><creatorcontrib>Rohde, Manfred</creatorcontrib><creatorcontrib>Deckwer, Wolf-Dieter</creatorcontrib><creatorcontrib>Jahn, Dieter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biedendieck, Rebekka</au><au>Gamer, Martin</au><au>Jaensch, Lothar</au><au>Meyer, Steffen</au><au>Rohde, Manfred</au><au>Deckwer, Wolf-Dieter</au><au>Jahn, Dieter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>132</volume><issue>4</issue><spage>426</spage><epage>430</epage><pages>426-430</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><abstract>A sucrose-inducible promoter system (P
sacB
) from
Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4
U
l
−1) and the heterologous green fluorescent protein GFP (7.9
mg
g
CDW
−1). Mutational analysis of
B. megaterium P
sacB
allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P
xylA
) for recombinant gene expression in
B. megaterium.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17692983</pmid><doi>10.1016/j.jbiotec.2007.07.494</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 2007-12, Vol.132 (4), p.426-430 |
| issn | 0168-1656 1873-4863 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68493870 |
| source | ScienceDirect Journals |
| subjects | Bacillus megaterium Bacillus megaterium - metabolism DNA Mutational Analysis Green Fluorescent Proteins - metabolism Hexosyltransferases - metabolism Promoter Regions, Genetic Recombinant protein production Recombinant Proteins - metabolism Sucrose - metabolism Sucrose-inducible promoter |
| title | A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium |
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