Identification, characterization and expression of a novel cytokine M17 homologue (MSH) in fish

Members of the interleukin 6 (IL6)-cytokine subfamily of proteins are involved in numerous physiological processes including cellular development, inflammatory function, and acute phase and immune responses. Previously, a cytokine-like gene named M17, which is closely associated with the IL6 subfami...

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Bibliographic Details
Published in:Fish & shellfish immunology 2007-12, Vol.23 (6), p.1256-1265
Main Authors: Hwang, Jee Youn, Santos, Mudjekeewis D., Kondo, Hidehiro, Hirono, Ikuo, Aoki, Takashi
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Amino Acid Sequence
Animals
Base Sequence
Brackish
Cytokine
Cytokines - chemistry
Cytokines - genetics
Danio rerio
Flounder - genetics
Flounder - immunology
Freshwater
Gene Expression Profiling - veterinary
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
Green spotted pufferfish
Japanese flounder
Marine
Molecular Sequence Data
MSH
Paralichthys olivaceus
Phylogeny
Pleuronectiformes
Poly I-C - pharmacology
Sequence Alignment - veterinary
Sequence Homology, Amino Acid
Stickleback
Synteny - genetics
Takifugu rubripes
Tetraodon nigroviridis
Tiger puffer
Zebrafish
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Summary:Members of the interleukin 6 (IL6)-cytokine subfamily of proteins are involved in numerous physiological processes including cellular development, inflammatory function, and acute phase and immune responses. Previously, a cytokine-like gene named M17, which is closely associated with the IL6 subfamily, has been identified in fish with no apparent orthologue in higher vertebrates. Here, we cloned a novel cDNA from Japanese flounder, Paralichthys olivaceus, which had significant identity but exhibited contrasting expression with fish M17s, named here as M17 Homologue (MSH). With subsequent in silico search and full annotation of the M17 orthologue in zebrafish ( Danio rerio), MSH orthologues in tiger puffer ( Takifugu rubripes), green spotted pufferfish ( Tetraodon nigroviridis) and stickleback ( Gastorosteus aculeatus), as well as structural, synteny comparisons and phylogenetic analysis with known IL6-cytokines, we determined the novelty of the fish MSH. Japanese flounder MSH was observed to be highly expressed in immune-related tissues and are induced by immune stimulants, lipopolysaccharide (LPS), polyI:C and peptidoglycan (PG) in vitro suggesting that it is involved in fish immunity particularly against viral and bacterial agents, a functional feature exhibited by previously reported fish cytokines.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2007.06.009