Mathematical modelling of human mesenchymal stem cell proliferation and differentiation inside artificial porous scaffolds

We present a mathematical model for the proliferation and differentiation of human mesenchymal stem cells grown inside artificial porous scaffolds under different oxygen concentrations. The values of parameters in the model are determined by comparison of the model solutions to published experimenta...

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Bibliographic Details
Published in:Journal of theoretical biology 2007-12, Vol.249 (3), p.543-553
Main Authors: Lemon, Greg, Waters, Sarah L., Rose, Felicity R.A.J., King, John R.
Format: Article
Language:English
Subjects:
Biocompatible Materials
Cell culture
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Hypoxia - physiology
Cell Proliferation - drug effects
Differential equations
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Humans
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Models, Biological
Oxygen - pharmacology
Regenerative medicine
Sensitivity analysis
Tissue engineering
Tissue Engineering - methods
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Summary:We present a mathematical model for the proliferation and differentiation of human mesenchymal stem cells grown inside artificial porous scaffolds under different oxygen concentrations. The values of parameters in the model are determined by comparison of the model solutions to published experimental data, complemented with a sensitivity analysis of the fitted parameters. It is shown that a simple hypothesis whereby the secretion of extra-cellular matrix (ECM) is oxygen dependent and that ECM itself stimulates cell proliferation is sufficient to explain the experimental data, which under conditions of low oxygen reveals increased total cell proliferation, upregulation of the numbers of undifferentiated cells, and extended lag phase. These results may help further to understand how cells proliferate inside artificial materials and are of importance to the field of tissue engineering.
ISSN:0022-5193
1095-8541
DOI:10.1016/j.jtbi.2007.08.015