Analysis of amelogenin gene (AMGX, AMGY) expression in ameloblastoma

Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detec...

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Bibliographic Details
Published in:Oral oncology 2005-09, Vol.41 (8), p.843-850
Main Authors: Tsujigiwa, Hidetsugu, Nagatsuka, Hitoshi, Han, Phuu Pwint, Gunduz, Mehmet, Siar, Chong Huat, Oida, Shinichiro, Nagai, Noriyuki
Format: Article
Language:English
Subjects:
Ameloblastoma
Ameloblastoma - genetics
Amelogenin
AMGX
AMGY
Biological and medical sciences
Chromosomes, Human, X
Chromosomes, Human, Y
Dental Enamel Proteins - genetics
Facial bones, jaws, teeth, parodontium: diseases, semeiology
Female
Gene Expression
Humans
In situ hybridization
Jaw Neoplasms - genetics
Male
Medical sciences
Otorhinolaryngology. Stomatology
Reverse Transcriptase Polymerase Chain Reaction
RT-PCR
Tumors
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Summary:Although the amelogenin gene is expressed in ameloblastoma, the precise expression pattern of X and Y amelogenin genes (AMGX, AMGY) in this tumor has not yet identified. In this study, we analyzed amelogenin gene expression in 19 samples (9 male, 10 female) of oral ameloblastomas by RT-PCR and detect the chromosomal origin of amelogenin mRNA by restriction enzyme digestion of the RT-PCR product. All tumor samples expressed amelogenin mRNA. We could detect increased level of AMGY expression in all male samples, higher than that of AMEX. It is an interesting finding as in normal male tooth development, the expression of AMGY is very much lower than that of AMGX. We postulate that epigenetic change of sex chromosomes may have some correlations with tumorigenesis of ameloblastoma. We also discuss the other possible mechanisms and points for future studies on this change in expression pattern.
ISSN:1368-8375
1879-0593
DOI:10.1016/j.oraloncology.2005.04.005