The Arabidopsis RNA-Binding Protein FCA Requires a Lysine-Specific Demethylase 1 Homolog to Downregulate FLC

A repressor of the transition to flowering in Arabidopsis is the MADS box protein FLOWERING LOCUS C (FLC). FCA, an RNA-binding protein, and FY, a homolog of the yeast RNA 3′ processing factor Pfs2p, downregulate FLC expression and therefore promote flowering. FCA/FY physically interact and alter pol...

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Bibliographic Details
Published in:Molecular cell 2007-11, Vol.28 (3), p.398-407
Main Authors: Liu, Fuquan, Quesada, Victor, Crevillén, Pedro, Bäurle, Isabel, Swiezewski, Szymon, Dean, Caroline
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
Chromatin Immunoprecipitation
DNA
Down-Regulation
Gene Silencing
Histone Deacetylases - chemistry
Histone Deacetylases - genetics
Histone Deacetylases - physiology
Histones - metabolism
MADS Domain Proteins - chemistry
MADS Domain Proteins - genetics
MADS Domain Proteins - metabolism
MADS Domain Proteins - physiology
Methylation
Molecular Sequence Data
Mutation
Ribonuclease III - metabolism
RNA
RNA Splicing
RNA, Messenger - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
RNA-Binding Proteins - physiology
Signal Transduction
Transcription, Genetic
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Summary:A repressor of the transition to flowering in Arabidopsis is the MADS box protein FLOWERING LOCUS C (FLC). FCA, an RNA-binding protein, and FY, a homolog of the yeast RNA 3′ processing factor Pfs2p, downregulate FLC expression and therefore promote flowering. FCA/FY physically interact and alter polyadenylation/3′ processing to negatively autoregulate FCA. Here, we show that FCA requires FLOWERING LOCUS D (FLD), a homolog of the human lysine-specific demethylase 1 (LSD1) for FLC downregulation. FCA also partially depends on DICER-LIKE 3, involved in chromatin silencing. fca mutations increased levels of unspliced sense FLC transcript, altered processing of antisense FLC transcripts, and increased H3K4 dimethylation in the central region of FLC. These data support a close association of FCA and FLD in mediating H3K4 demethylation and thus transcriptional silencing of FLC and reveal roles for antisense RNA processing and DCL3 function in this regulation.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2007.10.018