Fabrication, Characterization, and Application of Boron-Doped Diamond Microelectrodes for in Vivo Dopamine Detection

Highly boron-doped diamond (BDD) was deposited on chemically etched micrometer-sized tungsten wires using microwave plasma assisted chemical vapor deposition (MPCVD), and these were used to fabricate BDD microelectrodes. BDD microelectrodes with very small diameter (about 5 μm) and 250 μm in length...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-11, Vol.79 (22), p.8608-8615
Main Authors: Suzuki, Akane, Ivandini, Tribidasari A, Yoshimi, Kenji, Fujishima, Akira, Oyama, Genko, Nakazato, Taizo, Hattori, Nobutaka, Kitazawa, Shigeru, Einaga, Yasuaki
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Boron - chemistry
Brain
Chemical vapor deposition
Chemistry
Computer Simulation
Diamond
Diamonds
Dopamine - analysis
Electrochemical methods
Electrochemistry
Electrodes
Exact sciences and technology
Male
Mice
Microelectrodes
Microscopy, Electron, Scanning
Neurotransmitters
Oxidation-Reduction
Rodents
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Summary:Highly boron-doped diamond (BDD) was deposited on chemically etched micrometer-sized tungsten wires using microwave plasma assisted chemical vapor deposition (MPCVD), and these were used to fabricate BDD microelectrodes. BDD microelectrodes with very small diameter (about 5 μm) and 250 μm in length could be made successfully. In addition to the unique properties of BDD electrodes, such as a very low background current, high stability, and selective oxidation of dopamine (DA) in the presence of ascorbic acid (AA), other superior properties of the microelectrodes, including a constant current response, an increase in the mass transport, and the ability for use in high resistance media were also shown. An application study was conducted for in vivo detection of DA in mouse brain, where the BDD microelectrode was inserted into the corpus striatum of the mouse brain. A clear signal current response following medial forebrain bundle (MFB) stimulation could be obtained with high sensitivity. Excellent stability was achieved, indicating that the BDD microelectrodes are very promising for future in vivo electroanalysis.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac071519h