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Evidence for Unfolding and Refolding of Gas-Phase Cytochrome c Ions in a Paul Trap
The folding pathways of gas-phase cytochrome c ions produced by electrospray ionization have been studied by an ion trapping/ion mobility technique that allows conformations to be examined over extended timescales (10 ms to 10 s). The results show that the +9 charge state emerges from solution as a...
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| Published in: | Journal of the American Society for Mass Spectrometry 2005-09, Vol.16 (9), p.1493-1497 |
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| cites | cdi_FETCH-LOGICAL-c415t-bac6b95ff3354f011869b704ee51d158b2a4cf1c7329ac5fab1f9a88222964a23 |
| container_end_page | 1497 |
| container_issue | 9 |
| container_start_page | 1493 |
| container_title | Journal of the American Society for Mass Spectrometry |
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| creator | Badman, Ethan R. Myung, Sunnie Clemmer, David E. |
| description | The folding pathways of gas-phase cytochrome
c ions produced by electrospray ionization have been studied by an ion trapping/ion mobility technique that allows conformations to be examined over extended timescales (10 ms to 10 s). The results show that the +9 charge state emerges from solution as a compact structure and then rapidly unfolds into several substantially more open structures, a transition that requires 30–60 ms; over substantially longer timescales (250 ms to 10 s) elongated states appear to refold into an array of folded structures. The new folded states are less compact than those that are apparent during the initial unfolding. Apparently, unfolding to highly open conformations is a key step that must occur before +9 ions can sample more compact states that are stable at longer times. |
| doi_str_mv | 10.1016/j.jasms.2005.04.013 |
| format | article |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Biological and medical sciences Conformational dynamics in molecular biology Cytochrome Cytochromes c - chemistry Cytochromes c - ultrastructure Elongated structure Fundamental and applied biological sciences. Psychology Gases - chemistry Ionic mobility Ionization Ions Kinetics Mass spectrometry Molecular biophysics Phase Transition Protein Conformation Protein Folding Protein Renaturation Spectrometry, Mass, Electrospray Ionization - instrumentation Spectrometry, Mass, Electrospray Ionization - methods |
| title | Evidence for Unfolding and Refolding of Gas-Phase Cytochrome c Ions in a Paul Trap |
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