On-line preconcentration/determination of copper in parenteral solutions using activated carbon by inductively coupled plasma optical emission spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis 2005-09, Vol.39 (3), p.735-739
• Main Authors: Takara, E.A., Pasini-Cabello, S.D., Cerutti, S., Gásquez, J.A., Martinez, L.D.
• Format: Article
• Language: English
• Subjects: Absorption; Activated carbon; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; Calibration; Carbon - chemistry; Chemistry, Pharmaceutical - methods; Chromatography; Copper - analysis; Copper - chemistry; Copper determination; Fundamental and applied biological sciences. Psychology; General pharmacology; Hydrogen-Ion Concentration; ICP-OES; Infusions, Parenteral - instrumentation; Medical sciences; On-line preconcentration; Parenteral solutions; Pharmacology. Drug treatments; Reproducibility of Results; Risk; Sensitivity and Specificity; Spectrophotometry - methods; Spectrophotometry, Atomic - methods; Technology, Pharmaceutical; Time Factors
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2005.04.010

A method for the on-line preconcentration of copper using a minicolumn packed with activated carbon and its subsequent determination by inductively coupled plasma optical emission spectrometry (ICP-OES) coupled with flow injection (FI) was studied. In order to determinate the copper concentration present in parenteral solutions; it was retained on activated carbon (AC) at pH 9.5. A sensitivity enrichment factor of 30-fold was obtained with respect to the copper determination by ICP-OES without preconcentration. The detection limit for the preconcentration of 25 ml of sample was 0.1 μg l −1. The precision for the ten replicate determinations at the 2.5 μg l –1 Cu level was 3.0% relative standard deviation (R.S.D.), calculated with the peak heights. The calibration graph using the preconcentration method for cooper species was linear with a correlation coefficient of 0.9996 at levels near the detection limits up to at least 200 μg l −1. The method was successfully applied to the determination of copper in parenteral solutions.