Experimental studies in pigs on Trichinella detection in different diagnostic matrices

• Published in: Veterinary parasitology 2005-09, Vol.132 (1), p.85-90
• Main Authors: Nöckler, K., Serrano, F.J., Boireau, P., Kapel, C.M.O., Pozio, E.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Helminth - blood; antibody detection; Antigens, Helminth - chemistry; blood serum; disease detection; disease diagnosis; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Helminth Proteins - chemistry; inoculum density; Meat juice; meat juices; muscle juice; Muscle larvae; Muscle, Skeletal - parasitology; muscles; Pig; predilection sites; seroconversion; Serum; Specific Pathogen-Free Organisms; Swine; Swine Diseases - diagnosis; Swine Diseases - parasitology; Trichinella; Trichinella - growth & development; Trichinella detection; Trichinellosis - blood; Trichinellosis - parasitology; Trichinellosis - veterinary; trichinosis; worm recovery
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2005.05.033

A total of 72 specific pathogen-free (SPF) and Iberian pigs (three animals per group) were inoculated with 200, 1000 or 20,000 muscle larvae of T. spiralis, T. nativa, T. britovi and T. pseudospiralis. For each animal, the muscle larva burden was evaluated in nine muscle samples by digestion. The anti- Trichinella IgG kinetics in blood samples, taken twice prior and at days 5, 10, 15, 20, 25, 30, 40, 50 and 60 post-inoculation, and in muscle juice, obtained at necropsy, was evaluated by an ELISA using an excretory/secretory antigen. The mean larval recovery rate in SPF/Iberian pigs corresponded with the level of inoculum dose, and tongue, diaphragm and masseter were identified as predilection muscles. In SPF and Iberian pigs receiving 20,000 larvae of T. spiralis, an earlier seroconversion was detected from day 25 post-inoculation. At a 10-fold dilution, the muscle juice showed a good test agreement with blood serum.