Fluorometric investigation of the interaction of bovine serum albumin with surfactants and 6-mercaptopurine

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2005-09, Vol.80 (3), p.235-242
• Main Authors: Hu, Yan-Jun, Liu, Yi, Jiang, Wei, Zhao, Ru-Ming, Qu, Song-Sheng
• Format: Article
• Language: English
• Subjects: 6-Mercaptopurine; Bovine serum albumin; Cetrimonium Compounds; Fluorescence resonance energy transfer; Fluorescence spectroscopy; Indicators and Reagents; Ionic surfactant; Mercaptopurine; Serum Albumin, Bovine - chemistry; Spectrometry, Fluorescence - methods; Surface-Active Agents; UV–Visible spectroscopy
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2005.04.005

Fluorescence quenching in solutions of bovine serum albumin has been investigated in the presence of 6-mercaptopurine and ionic surfactants. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of bovine serum albumin by 6-mercaptopurine was dynamic quenching mechanism. The Stern–Volmer quenching model has been successfully applied, and the activation energy of the interaction between 6-mercaptopurine and bovine serum albumin as much as 4.26 kJ mol −1 was calculated. The distance r between donor (bovine serum albumin) and acceptor (6-mercaptopurine) was obtained according to fluorescence resonance energy transfer (FRET). The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine.