Effect of Lifelong Coenzyme Q10 Supplementation on Age-Related Oxidative Stress and Mitochondrial Function in Liver and Skeletal Muscle of Rats Fed on a Polyunsaturated Fatty Acid (PUFA)-Rich Diet

• Published in: The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2007-11, Vol.62 (11), p.1211-1218
• Main Authors: Ochoa, Julio J., Quiles, José L., López-Frías, Magdalena, Huertas, Jesús R., Mataix, José
• Format: Article
• Language: English
• Subjects: Aging - drug effects; Aging - metabolism; alpha-Tocopherol - metabolism; Animals; Catalase - metabolism; Coenzymes - administration & dosage; Coenzymes - pharmacology; Dietary Fats, Unsaturated - administration & dosage; Electron Transport Complex IV - metabolism; Fatty Acids, Unsaturated - administration & dosage; Lipid Peroxidation; Male; Mitochondria, Liver - drug effects; Mitochondria, Liver - metabolism; Mitochondria, Muscle - metabolism; Muscle, Skeletal - drug effects; Oxidative Stress - drug effects; Rats; Rats, Wistar; Ubiquinone - administration & dosage; Ubiquinone - analogs & derivatives; Ubiquinone - pharmacology
• ISSN: 1079-5006; 1758-535X
• DOI: 10.1093/gerona/62.11.1211

This study investigates aging-related changes in lipid peroxidation and functionality in liver and skeletal-muscle mitochondria in rats fed a diet rich in polyunsaturated fatty acids (PUFA), depending on supplementation or not with coenzyme Q10 (CoQ10). Two groups of rats were fed for 24 months on a PUFA-rich diet, differing in supplementation or not with CoQ10. At 6 and 24 months mitochondria were analyzed for fatty acid profile; hydroperoxides; α-tocopherol; CoQ9; CoQ10; cytochromes b, c+c1, and a+a3 contents; cytochrome c oxidase activity; and catalase activity in cytosol. Results of this study showed for the supplemented group an age-associated decrease in the peroxidizability index, an increase in catalase activity in skeletal muscle, and modulation of the aging-related changes in different mitochondrial electron-transport-chain components in skeletal muscle. These findings provide mechanisms to explain the effect of CoQ10 in extending the life span of animals fed a PUFA-rich diet.