Loading…
Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro
Abstract Introduction Tumor refractoriness to chemotherapy is frequently due to the acquisition of resistance. Resistant cells selected by exposure to chemotherapy agents may exhibit differences in [18 F]fluoro-2-deoxy- d -glucose (FDG) incorporation, as compared with sensitive cells. Methods FDG in...
Saved in:
Published in: | Nuclear medicine and biology 2007-11, Vol.34 (8), p.955-960 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123 |
---|---|
cites | cdi_FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123 |
container_end_page | 960 |
container_issue | 8 |
container_start_page | 955 |
container_title | Nuclear medicine and biology |
container_volume | 34 |
creator | Smith, Tim A.D Sharma, Rituka I Wang, Weiguang G Welch, Andy E Schweiger, Lutz F Collie-Duguid, Elaina S.R |
description | Abstract Introduction Tumor refractoriness to chemotherapy is frequently due to the acquisition of resistance. Resistant cells selected by exposure to chemotherapy agents may exhibit differences in [18 F]fluoro-2-deoxy- d -glucose (FDG) incorporation, as compared with sensitive cells. Methods FDG incorporation, hexokinase (HK) activity, glucose transport and ATP content were determined in clones of 5-fluorouracil (5FU)-resistant MCF7 cells, established by long-term exposure to increasing 5FU concentrations, and in parental MCF7 cells. Results FDG incorporation was decreased in MCF7 cells resistant to 5FU; HK activity was similar in the resistant and sensitive cells, while glucose transport was increased, as compared with sensitive cells. Treatment of cells with the glucose efflux inhibitor phloretin increased FDG incorporation to similar levels in the resistant and sensitive cells. Analysis of microarray data demonstrated the expression of GLUT1, 8 and 10 transporters in MCF7 cells. GLUT8 and 10 expression was decreased in the resistant cells, while GLUT1 was only increased in cells resistant to the lowest 5FU concentration. Conclusion FDG incorporation in 5FU-resistant MCF7 cells is decreased, as compared with sensitive cells. Our findings also suggest that this may be due to high rates of membrane glucose transport in the resistant cells resulting in enhanced efflux of FDG. We believe that this is the first demonstration that facilitative glucose transporters can actually decrease the incorporation of FDG. |
doi_str_mv | 10.1016/j.nucmedbio.2007.07.007 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68506293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0969805107001874</els_id><sourcerecordid>68506293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123</originalsourceid><addsrcrecordid>eNqNks9uEzEQxi0EoqHwCuATtw3jza7_XJCqQACpFQfoCSHLa8-Cw2YdbG8hD9L3xauEInGqNJJl-_fN2PMNIS8YLBkw_mq7HCe7Q9f5sKwBxHIOEA_IgklRV4qz5iFZgOKqktCyM_IkpS0UZcPgMTljQikJSi7I7Ru0EU1CR78wSTdf-2EKMVR15TD8PlTU0erbMNmQkPrRhrgP0WQfRmpGN5-cxH-ZHM2YCpOpiUhNSsF6kwvwy-fvNGLyKZvRFjDQdnNdMtCr9UZQi8OQ5t2NzzE8JY96MyR8dlrPyfXm7ef1--ry47sP64vLyjZ1kytuRd8ahVBCuga4NGga50TXy45zsB3vAVZO1b3hplG8l0Ia0yoGtXWsXp2Tl8e8-xh-Tpiy3vk0P8WMGKakuWyB12pVQHEEbQwpRez1PvqdiQfNQM-O6K2-c0TPjug5QBTl81OJqSvX_3QnCwpwcQSwfPTGY9TJeiwtcj6izdoFf48ir__LYQc_emuGH3jAtA1THEsfNdOp1qA_zYMxzwWIMhNSNKs_0GS3tw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68506293</pqid></control><display><type>article</type><title>Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro</title><source>Elsevier</source><creator>Smith, Tim A.D ; Sharma, Rituka I ; Wang, Weiguang G ; Welch, Andy E ; Schweiger, Lutz F ; Collie-Duguid, Elaina S.R</creator><creatorcontrib>Smith, Tim A.D ; Sharma, Rituka I ; Wang, Weiguang G ; Welch, Andy E ; Schweiger, Lutz F ; Collie-Duguid, Elaina S.R</creatorcontrib><description>Abstract Introduction Tumor refractoriness to chemotherapy is frequently due to the acquisition of resistance. Resistant cells selected by exposure to chemotherapy agents may exhibit differences in [18 F]fluoro-2-deoxy- d -glucose (FDG) incorporation, as compared with sensitive cells. Methods FDG incorporation, hexokinase (HK) activity, glucose transport and ATP content were determined in clones of 5-fluorouracil (5FU)-resistant MCF7 cells, established by long-term exposure to increasing 5FU concentrations, and in parental MCF7 cells. Results FDG incorporation was decreased in MCF7 cells resistant to 5FU; HK activity was similar in the resistant and sensitive cells, while glucose transport was increased, as compared with sensitive cells. Treatment of cells with the glucose efflux inhibitor phloretin increased FDG incorporation to similar levels in the resistant and sensitive cells. Analysis of microarray data demonstrated the expression of GLUT1, 8 and 10 transporters in MCF7 cells. GLUT8 and 10 expression was decreased in the resistant cells, while GLUT1 was only increased in cells resistant to the lowest 5FU concentration. Conclusion FDG incorporation in 5FU-resistant MCF7 cells is decreased, as compared with sensitive cells. Our findings also suggest that this may be due to high rates of membrane glucose transport in the resistant cells resulting in enhanced efflux of FDG. We believe that this is the first demonstration that facilitative glucose transporters can actually decrease the incorporation of FDG.</description><identifier>ISSN: 0969-8051</identifier><identifier>EISSN: 1872-9614</identifier><identifier>DOI: 10.1016/j.nucmedbio.2007.07.007</identifier><identifier>PMID: 17998098</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biological Transport, Active - drug effects ; Breast Neoplasms - diagnostic imaging ; Breast Neoplasms - metabolism ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; FDG ; Fluorodeoxyglucose F18 - pharmacokinetics ; Fluorouracil - administration & dosage ; Glucose - metabolism ; Glucose transport ; Humans ; MCF7 cells ; Metabolic Clearance Rate - drug effects ; Radiology ; Radionuclide Imaging ; Radiopharmaceuticals - pharmacokinetics</subject><ispartof>Nuclear medicine and biology, 2007-11, Vol.34 (8), p.955-960</ispartof><rights>Elsevier Inc.</rights><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123</citedby><cites>FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17998098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Tim A.D</creatorcontrib><creatorcontrib>Sharma, Rituka I</creatorcontrib><creatorcontrib>Wang, Weiguang G</creatorcontrib><creatorcontrib>Welch, Andy E</creatorcontrib><creatorcontrib>Schweiger, Lutz F</creatorcontrib><creatorcontrib>Collie-Duguid, Elaina S.R</creatorcontrib><title>Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro</title><title>Nuclear medicine and biology</title><addtitle>Nucl Med Biol</addtitle><description>Abstract Introduction Tumor refractoriness to chemotherapy is frequently due to the acquisition of resistance. Resistant cells selected by exposure to chemotherapy agents may exhibit differences in [18 F]fluoro-2-deoxy- d -glucose (FDG) incorporation, as compared with sensitive cells. Methods FDG incorporation, hexokinase (HK) activity, glucose transport and ATP content were determined in clones of 5-fluorouracil (5FU)-resistant MCF7 cells, established by long-term exposure to increasing 5FU concentrations, and in parental MCF7 cells. Results FDG incorporation was decreased in MCF7 cells resistant to 5FU; HK activity was similar in the resistant and sensitive cells, while glucose transport was increased, as compared with sensitive cells. Treatment of cells with the glucose efflux inhibitor phloretin increased FDG incorporation to similar levels in the resistant and sensitive cells. Analysis of microarray data demonstrated the expression of GLUT1, 8 and 10 transporters in MCF7 cells. GLUT8 and 10 expression was decreased in the resistant cells, while GLUT1 was only increased in cells resistant to the lowest 5FU concentration. Conclusion FDG incorporation in 5FU-resistant MCF7 cells is decreased, as compared with sensitive cells. Our findings also suggest that this may be due to high rates of membrane glucose transport in the resistant cells resulting in enhanced efflux of FDG. We believe that this is the first demonstration that facilitative glucose transporters can actually decrease the incorporation of FDG.</description><subject>Biological Transport, Active - drug effects</subject><subject>Breast Neoplasms - diagnostic imaging</subject><subject>Breast Neoplasms - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Drug Resistance, Neoplasm</subject><subject>FDG</subject><subject>Fluorodeoxyglucose F18 - pharmacokinetics</subject><subject>Fluorouracil - administration & dosage</subject><subject>Glucose - metabolism</subject><subject>Glucose transport</subject><subject>Humans</subject><subject>MCF7 cells</subject><subject>Metabolic Clearance Rate - drug effects</subject><subject>Radiology</subject><subject>Radionuclide Imaging</subject><subject>Radiopharmaceuticals - pharmacokinetics</subject><issn>0969-8051</issn><issn>1872-9614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNks9uEzEQxi0EoqHwCuATtw3jza7_XJCqQACpFQfoCSHLa8-Cw2YdbG8hD9L3xauEInGqNJJl-_fN2PMNIS8YLBkw_mq7HCe7Q9f5sKwBxHIOEA_IgklRV4qz5iFZgOKqktCyM_IkpS0UZcPgMTljQikJSi7I7Ru0EU1CR78wSTdf-2EKMVR15TD8PlTU0erbMNmQkPrRhrgP0WQfRmpGN5-cxH-ZHM2YCpOpiUhNSsF6kwvwy-fvNGLyKZvRFjDQdnNdMtCr9UZQi8OQ5t2NzzE8JY96MyR8dlrPyfXm7ef1--ry47sP64vLyjZ1kytuRd8ahVBCuga4NGga50TXy45zsB3vAVZO1b3hplG8l0Ia0yoGtXWsXp2Tl8e8-xh-Tpiy3vk0P8WMGKakuWyB12pVQHEEbQwpRez1PvqdiQfNQM-O6K2-c0TPjug5QBTl81OJqSvX_3QnCwpwcQSwfPTGY9TJeiwtcj6izdoFf48ir__LYQc_emuGH3jAtA1THEsfNdOp1qA_zYMxzwWIMhNSNKs_0GS3tw</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Smith, Tim A.D</creator><creator>Sharma, Rituka I</creator><creator>Wang, Weiguang G</creator><creator>Welch, Andy E</creator><creator>Schweiger, Lutz F</creator><creator>Collie-Duguid, Elaina S.R</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro</title><author>Smith, Tim A.D ; Sharma, Rituka I ; Wang, Weiguang G ; Welch, Andy E ; Schweiger, Lutz F ; Collie-Duguid, Elaina S.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological Transport, Active - drug effects</topic><topic>Breast Neoplasms - diagnostic imaging</topic><topic>Breast Neoplasms - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Drug Resistance, Neoplasm</topic><topic>FDG</topic><topic>Fluorodeoxyglucose F18 - pharmacokinetics</topic><topic>Fluorouracil - administration & dosage</topic><topic>Glucose - metabolism</topic><topic>Glucose transport</topic><topic>Humans</topic><topic>MCF7 cells</topic><topic>Metabolic Clearance Rate - drug effects</topic><topic>Radiology</topic><topic>Radionuclide Imaging</topic><topic>Radiopharmaceuticals - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Tim A.D</creatorcontrib><creatorcontrib>Sharma, Rituka I</creatorcontrib><creatorcontrib>Wang, Weiguang G</creatorcontrib><creatorcontrib>Welch, Andy E</creatorcontrib><creatorcontrib>Schweiger, Lutz F</creatorcontrib><creatorcontrib>Collie-Duguid, Elaina S.R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nuclear medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Tim A.D</au><au>Sharma, Rituka I</au><au>Wang, Weiguang G</au><au>Welch, Andy E</au><au>Schweiger, Lutz F</au><au>Collie-Duguid, Elaina S.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro</atitle><jtitle>Nuclear medicine and biology</jtitle><addtitle>Nucl Med Biol</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>34</volume><issue>8</issue><spage>955</spage><epage>960</epage><pages>955-960</pages><issn>0969-8051</issn><eissn>1872-9614</eissn><abstract>Abstract Introduction Tumor refractoriness to chemotherapy is frequently due to the acquisition of resistance. Resistant cells selected by exposure to chemotherapy agents may exhibit differences in [18 F]fluoro-2-deoxy- d -glucose (FDG) incorporation, as compared with sensitive cells. Methods FDG incorporation, hexokinase (HK) activity, glucose transport and ATP content were determined in clones of 5-fluorouracil (5FU)-resistant MCF7 cells, established by long-term exposure to increasing 5FU concentrations, and in parental MCF7 cells. Results FDG incorporation was decreased in MCF7 cells resistant to 5FU; HK activity was similar in the resistant and sensitive cells, while glucose transport was increased, as compared with sensitive cells. Treatment of cells with the glucose efflux inhibitor phloretin increased FDG incorporation to similar levels in the resistant and sensitive cells. Analysis of microarray data demonstrated the expression of GLUT1, 8 and 10 transporters in MCF7 cells. GLUT8 and 10 expression was decreased in the resistant cells, while GLUT1 was only increased in cells resistant to the lowest 5FU concentration. Conclusion FDG incorporation in 5FU-resistant MCF7 cells is decreased, as compared with sensitive cells. Our findings also suggest that this may be due to high rates of membrane glucose transport in the resistant cells resulting in enhanced efflux of FDG. We believe that this is the first demonstration that facilitative glucose transporters can actually decrease the incorporation of FDG.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17998098</pmid><doi>10.1016/j.nucmedbio.2007.07.007</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0969-8051 |
ispartof | Nuclear medicine and biology, 2007-11, Vol.34 (8), p.955-960 |
issn | 0969-8051 1872-9614 |
language | eng |
recordid | cdi_proquest_miscellaneous_68506293 |
source | Elsevier |
subjects | Biological Transport, Active - drug effects Breast Neoplasms - diagnostic imaging Breast Neoplasms - metabolism Cell Line, Tumor Drug Resistance, Neoplasm FDG Fluorodeoxyglucose F18 - pharmacokinetics Fluorouracil - administration & dosage Glucose - metabolism Glucose transport Humans MCF7 cells Metabolic Clearance Rate - drug effects Radiology Radionuclide Imaging Radiopharmaceuticals - pharmacokinetics |
title | Decreased [18 F]fluoro-2-deoxy- d -glucose incorporation and increased glucose transport are associated with resistance to 5FU in MCF7 cells in vitro |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T15%3A15%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Decreased%20%5B18%20F%5Dfluoro-2-deoxy-%20d%20-glucose%20incorporation%20and%20increased%20glucose%20transport%20are%20associated%20with%20resistance%20to%205FU%20in%20MCF7%20cells%20in%20vitro&rft.jtitle=Nuclear%20medicine%20and%20biology&rft.au=Smith,%20Tim%20A.D&rft.date=2007-11-01&rft.volume=34&rft.issue=8&rft.spage=955&rft.epage=960&rft.pages=955-960&rft.issn=0969-8051&rft.eissn=1872-9614&rft_id=info:doi/10.1016/j.nucmedbio.2007.07.007&rft_dat=%3Cproquest_cross%3E68506293%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c424t-6c7f5a9e09e08d4068aea4dd7bf8b660cb6f003d92fa6a496f878aa59102cd123%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=68506293&rft_id=info:pmid/17998098&rfr_iscdi=true |