Determination of tocopherols in vegetable oils by CEC using methacrylate ester-based monolithic columns

The separation and determination of tocopherols (Ts) in vegetable oils by CEC using methacrylate ester-based monolithic columns has been developed. The effects of pore size of the monolithic columns were studied, and the composition of mobile phase was optimized. The optimal pore size of the monolit...

Full description

Saved in:
Bibliographic Details
Published in:Electrophoresis 2007-11, Vol.28 (22), p.4128-4135
Main Authors: Lerma-García, María Jesús, Simó-Alfonso, Ernesto F, Ramis-Ramos, Guillermo, Herrero-Martínez, José Manuel
Format: Article
Language:English
Subjects:
CEC
Chromatography, Micellar Electrokinetic Capillary - instrumentation
Chromatography, Micellar Electrokinetic Capillary - methods
Chromatography, Micellar Electrokinetic Capillary - standards
Food analysis
Methacrylate ester-based monolithic column
Methacrylates
Olive Oil
Palm Oil
Plant Oils - chemistry
Plant Oils - standards
Seeds - chemistry
Tocopherols
Tocopherols - analysis
Tocotrienols - analysis
Vegetable oils
Vitis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The separation and determination of tocopherols (Ts) in vegetable oils by CEC using methacrylate ester-based monolithic columns has been developed. The effects of pore size of the monolithic columns were studied, and the composition of mobile phase was optimized. The optimal pore size of the monolith was obtained with 12 wt% 1,4-butanediol in the polymerization mixture. Excellent resolution between tocopherols was achieved within 10 min analysis time with a 99:1 v/v MeOH-aqueous buffer containing 5 mM tris(hydroxymethyl)aminomethane at pH 8.0. The LODs were lower than 2.3 μg/mL, and interday and column-to-column reproducibilities at 25 μg/mL were better than 5.6%. Using a 93:7 v/v MeOH-aqueous buffer, both tocopherols and tocotrienols (T₃s) of grapeseed and palm oils were resolved. Application to the detection of olive oil adulteration with low-cost edible oils was demonstrated.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200700195