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Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus
Hepatitis B virus (HBV) is a hepadnavirus that is a major cause of acute and chronic hepatitis in humans. Hepatitis B viral infection itself is noncytopathic, and it is the immune response to the viral antigens that is thought to be responsible for hepatic pathology. Previously, we developed a trans...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 2007-11, Vol.104 (46), p.18187-18192 |
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creator | Vilarinho, Sílvia Ogasawara, Kouetsu Nishimura, Stephen Lanier, Lewis L Baron, Jody L |
description | Hepatitis B virus (HBV) is a hepadnavirus that is a major cause of acute and chronic hepatitis in humans. Hepatitis B viral infection itself is noncytopathic, and it is the immune response to the viral antigens that is thought to be responsible for hepatic pathology. Previously, we developed a transgenic mouse model of primary HBV infection and demonstrated that the acute liver injury is mediated by nonclassical natural killer (NK)T cells, which are CD1d-restricted, but nonreactive to α-GalCer. We now demonstrate a role for NKG2D and its ligands in this nonclassical NKT cell-mediated immune response to hepatitis B virus and in the subsequent acute hepatitis that ensues. Surface expression of NKG2D and one of its ligands (retinoic acid early inducible-1 or RAE-1) are modulated in an HBV-dependent manner. Furthermore, blockade of an NKG2D-ligand interaction completely prevents the HBV- and CD1d-dependent, nonclassical NKT cell-mediated acute hepatitis and liver injury. This study has major implications for understanding activation of NKT cells and identifies a potential therapeutic target in treating hepatitis B viral infection. |
doi_str_mv | 10.1073/pnas.0708968104 |
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Hepatitis B viral infection itself is noncytopathic, and it is the immune response to the viral antigens that is thought to be responsible for hepatic pathology. Previously, we developed a transgenic mouse model of primary HBV infection and demonstrated that the acute liver injury is mediated by nonclassical natural killer (NK)T cells, which are CD1d-restricted, but nonreactive to α-GalCer. We now demonstrate a role for NKG2D and its ligands in this nonclassical NKT cell-mediated immune response to hepatitis B virus and in the subsequent acute hepatitis that ensues. Surface expression of NKG2D and one of its ligands (retinoic acid early inducible-1 or RAE-1) are modulated in an HBV-dependent manner. Furthermore, blockade of an NKG2D-ligand interaction completely prevents the HBV- and CD1d-dependent, nonclassical NKT cell-mediated acute hepatitis and liver injury. This study has major implications for understanding activation of NKT cells and identifies a potential therapeutic target in treating hepatitis B viral infection.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0708968104</identifier><identifier>PMID: 17991774</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Adoptive transfer ; Animals ; Antigens ; Biological Sciences ; Cell Line ; Cells ; Gene expression ; Hepadnavirus ; Hepatitis ; Hepatitis B ; Hepatitis B - prevention & control ; Hepatitis B virus ; Hepatitis B virus - immunology ; Hepatitis B virus - physiology ; Hepatocytes ; Humans ; Infections ; Killer Cells, Natural - immunology ; Liver ; Mice ; Mice, Transgenic ; Natural killer cells ; Natural killer T cells ; NK Cell Lectin-Like Receptor Subfamily K ; Receptors, Immunologic - antagonists & inhibitors ; Receptors, Natural Killer Cell ; Rodents ; Splenocytes ; T lymphocytes ; Virus Replication ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2007-11, Vol.104 (46), p.18187-18192</ispartof><rights>Copyright 2007 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 13, 2007</rights><rights>2007 by The National Academy of Sciences of the USA 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c620t-2da5dae3200a5ab93468dbb98e99047846ecd1a26de71aa33d038aad3b7f6e963</citedby><cites>FETCH-LOGICAL-c620t-2da5dae3200a5ab93468dbb98e99047846ecd1a26de71aa33d038aad3b7f6e963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/104/46.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25450395$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25450395$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17991774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vilarinho, Sílvia</creatorcontrib><creatorcontrib>Ogasawara, Kouetsu</creatorcontrib><creatorcontrib>Nishimura, Stephen</creatorcontrib><creatorcontrib>Lanier, Lewis L</creatorcontrib><creatorcontrib>Baron, Jody L</creatorcontrib><title>Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Hepatitis B virus (HBV) is a hepadnavirus that is a major cause of acute and chronic hepatitis in humans. Hepatitis B viral infection itself is noncytopathic, and it is the immune response to the viral antigens that is thought to be responsible for hepatic pathology. Previously, we developed a transgenic mouse model of primary HBV infection and demonstrated that the acute liver injury is mediated by nonclassical natural killer (NK)T cells, which are CD1d-restricted, but nonreactive to α-GalCer. We now demonstrate a role for NKG2D and its ligands in this nonclassical NKT cell-mediated immune response to hepatitis B virus and in the subsequent acute hepatitis that ensues. Surface expression of NKG2D and one of its ligands (retinoic acid early inducible-1 or RAE-1) are modulated in an HBV-dependent manner. Furthermore, blockade of an NKG2D-ligand interaction completely prevents the HBV- and CD1d-dependent, nonclassical NKT cell-mediated acute hepatitis and liver injury. This study has major implications for understanding activation of NKT cells and identifies a potential therapeutic target in treating hepatitis B viral infection.</description><subject>Adoptive transfer</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biological Sciences</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Gene expression</subject><subject>Hepadnavirus</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B - prevention & control</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - immunology</subject><subject>Hepatitis B virus - physiology</subject><subject>Hepatocytes</subject><subject>Humans</subject><subject>Infections</subject><subject>Killer Cells, Natural - immunology</subject><subject>Liver</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Natural killer cells</subject><subject>Natural killer T cells</subject><subject>NK Cell Lectin-Like Receptor Subfamily K</subject><subject>Receptors, Immunologic - antagonists & inhibitors</subject><subject>Receptors, Natural Killer Cell</subject><subject>Rodents</subject><subject>Splenocytes</subject><subject>T lymphocytes</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFks1v1DAQxSMEokvhzAmwOCBxSDv-iD8uSLRAQVRwoD1bTux0syRxsJ0V_Pc42lW3cOnJluY3T_PmTVE8x3CCQdDTaTTxBARIxSUG9qBYYVC45EzBw2IFQEQpGWFHxZMYNwCgKgmPiyMslMJCsFVhz3rf_DTWId-ib18vyAfkx_y5Qo3r-4im4LZuTBGt3WRSl7qIzGhRWjtkmjk51A3DPDoUXJz8GB1K_g56hrZdmOPT4lFr-uie7d_j4vrTx6vzz-Xl94sv5-8vy4YTSCWxprLGUQJgKlMryri0da2kUwqYkIy7xmJDuHUCG0OpBSqNsbQWLXeK0-Pi3U53muvB2SYPHkyvp9ANJvzR3nT638rYrfWN32oCklEss8CbvUDwv2YXkx66uCzCjM7PUXNZgcBU3AsSoEJQqjL4-j9w4-cw5i1kBlMuQZEMne6gJvgYg2tvR8agl5z1krM-5Jw7Xt51euD3wWYA7YGl8yDHNOMaSywXD2_vQXQ7931yv1NmX-zYTUw-3MKkYhVQVeX6q129NV6bm9BFff1jMQggicyHR_8CJVjPog</recordid><startdate>20071113</startdate><enddate>20071113</enddate><creator>Vilarinho, Sílvia</creator><creator>Ogasawara, Kouetsu</creator><creator>Nishimura, Stephen</creator><creator>Lanier, Lewis L</creator><creator>Baron, Jody L</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20071113</creationdate><title>Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus</title><author>Vilarinho, Sílvia ; Ogasawara, Kouetsu ; Nishimura, Stephen ; Lanier, Lewis L ; Baron, Jody L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c620t-2da5dae3200a5ab93468dbb98e99047846ecd1a26de71aa33d038aad3b7f6e963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adoptive transfer</topic><topic>Animals</topic><topic>Antigens</topic><topic>Biological Sciences</topic><topic>Cell Line</topic><topic>Cells</topic><topic>Gene expression</topic><topic>Hepadnavirus</topic><topic>Hepatitis</topic><topic>Hepatitis B</topic><topic>Hepatitis B - prevention & control</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - immunology</topic><topic>Hepatitis B virus - physiology</topic><topic>Hepatocytes</topic><topic>Humans</topic><topic>Infections</topic><topic>Killer Cells, Natural - immunology</topic><topic>Liver</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Natural killer cells</topic><topic>Natural killer T cells</topic><topic>NK Cell Lectin-Like Receptor Subfamily K</topic><topic>Receptors, Immunologic - antagonists & inhibitors</topic><topic>Receptors, Natural Killer Cell</topic><topic>Rodents</topic><topic>Splenocytes</topic><topic>T lymphocytes</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilarinho, Sílvia</creatorcontrib><creatorcontrib>Ogasawara, Kouetsu</creatorcontrib><creatorcontrib>Nishimura, Stephen</creatorcontrib><creatorcontrib>Lanier, Lewis L</creatorcontrib><creatorcontrib>Baron, Jody L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilarinho, Sílvia</au><au>Ogasawara, Kouetsu</au><au>Nishimura, Stephen</au><au>Lanier, Lewis L</au><au>Baron, Jody L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2007-11-13</date><risdate>2007</risdate><volume>104</volume><issue>46</issue><spage>18187</spage><epage>18192</epage><pages>18187-18192</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Hepatitis B virus (HBV) is a hepadnavirus that is a major cause of acute and chronic hepatitis in humans. 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This study has major implications for understanding activation of NKT cells and identifies a potential therapeutic target in treating hepatitis B viral infection.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>17991774</pmid><doi>10.1073/pnas.0708968104</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | JSTOR Archival Journals; PubMed Central |
subjects | Adoptive transfer Animals Antigens Biological Sciences Cell Line Cells Gene expression Hepadnavirus Hepatitis Hepatitis B Hepatitis B - prevention & control Hepatitis B virus Hepatitis B virus - immunology Hepatitis B virus - physiology Hepatocytes Humans Infections Killer Cells, Natural - immunology Liver Mice Mice, Transgenic Natural killer cells Natural killer T cells NK Cell Lectin-Like Receptor Subfamily K Receptors, Immunologic - antagonists & inhibitors Receptors, Natural Killer Cell Rodents Splenocytes T lymphocytes Virus Replication Viruses |
title | Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus |
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