Chemical or microbiological models of secondary caries development around different dental restorative materials

• Published in: Journal of biomedical materials research 2005-08, Vol.74B (2), p.725-731
• Main Authors: Lobo, Maristela M., Gonçalves, Reginaldo B., Ambrosano, Gláucia Maria B., Pimenta, Luiz André F.
• Format: Article
• Language: English
• Subjects: Animals; caries models; Cattle; Dental Caries - microbiology; Dental Materials - chemistry; Dental Restoration, Permanent; Disease Models, Animal; fluoride; Hardness; Microscopy, Polarization; Models, Biological; Models, Chemical; pH cycling; secondary caries; Streptococcus mutans
• ISSN: 1552-4973; 0021-9304; 1552-4981
• DOI: 10.1002/jbm.b.30253

This study evaluated artificial secondary caries around restorative materials, induced by means of chemical or microbiological models. The following materials were used randomly to restore 130 dental blocks: (1) zinc‐oxide eugenol‐free temporary filling: Coltosol (Coltène/Whaledent Inc.; n = 30), (2) silver amalgam: Permite C (SDI Limited, n = 20), (3) composite resin: Filtek™ Z250 (3M ESPE; n = 20), (4) glass‐ionomer cement: Fuji II (GC America Inc.; n = 20), (5) resin‐modified glass ionomer: Vitremer™ (3M ESPE; n = 20), and (6) polyacid modified resin: Dyract AP (Dentsply; n = 20). Ten specimens of Group 1 were kept in humidity, and had no carious formation (NC). Ten specimens of each group were submitted to pH cycling (CG, n = 60), and the others were immersed in a medium containing Streptococcus mutans and sucrose (BG, n = 60). Mineral content was determined by microhardness assessment, and lesion depth was measured in polarized light photomicrographs. In the chemical model (CG), mineral content values in the vicinities of restoration were high for Groups 5 (75.7 ± 11.9), 4 (70.8 ± 14.2), and NC (95.4 ± 3.8); intermediate for Groups 1 (55.8 ± 18.5), 6 (45.6 ± 11.0), and 2 (44.3 ± 11.2); and reduced for Group 3 (34.7 ± 9.7). In the microbiological model (BG), results were similar to CG, although there was less demineralization. The highest lesion depths were found for Groups 3 (182.3 ± 33.2) in CG and 6 (126.5 ± 42.8) in BG, when compared to Group 5 (114.6 ± 26.0 and 56.2 ± 33.2, respectively). In both models of caries induction, ionomeric materials showed a superior cariostatic effect when compared to the other restorative materials. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005