Effects of tumor necrosis factor-α on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro

• Published in: Journal of experimental zoology. Part A, Ecological genetics and physiology Ecological genetics and physiology, 2007-12, Vol.307A (12), p.699-707
• Main Authors: Gamo, Toru, Yamauchi, Nobuhiko, Nishimura, Kyohei, Watanabe, Ryo, Matsumoto, Kenji, Oozono, Shinji, Kubota, Kaiyu, He, Pei-Jian, Soh, Tomoki, Hattori, Masa-aki
• Format: Article
• Language: English
• Subjects: Animals; Cell Proliferation - drug effects; Cells, Cultured; Dinoprost - metabolism; Dinoprostone - metabolism; Dose-Response Relationship, Drug; Embryo, Mammalian - cytology; Endometrium - drug effects; Endometrium - metabolism; Endometrium - pathology; Epidermal Growth Factor - pharmacology; Female; Fibroblast Growth Factor 2 - pharmacology; Male; Matrix Metalloproteinases - metabolism; Pregnancy; Rats; Rats, Wistar; Stromal Cells - drug effects; Stromal Cells - metabolism; Stromal Cells - pathology; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 1932-5223; 1932-5231
• DOI: 10.1002/jez.a.423

Tumor necrosis factor‐α (TNF‐α) is known as a pluripotent cell mediator, and it is implicated in the control of uterine cell growth, differentiation and function during estrous cycle and pregnancy. In this study, we investigated the effect of TNF‐α on endometrial stromal cells derived from rat uterus (rat endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 of pregnancy. Proliferation activities of RES were measured by using bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) were measured by enzyme immunoassay kits and the production of matrix metalloproteinases (MMPs) was analyzed by gelatin‐zymography. TNF‐α, as well as epidermal growth factor and fibroblast growth factor‐2, significantly increased the proliferation activity of RES (P